Pereira Cíntia A M, Yariwake Janete H, McCullagh Michael
Universidade de São Paulo, Instituto de Química de São Carlos, São Carlos, SP, Brazil.
Phytochem Anal. 2005 Sep-Oct;16(5):295-301. doi: 10.1002/pca.820.
HPLC-MS using collision induced dissociation (CID) has been utilised for the identification of the C-glycosylflavone isomer pairs orientin/isoorientin and vitexin/isovitexin. HPLC-CID/MS analyses produced pseudo-MS/MS spectra that allowed the identification of the flavone C-glycosides. The efficient differentiation of isomers was performed by comparing the CID-MS/MS spectra (including exact mass measurements) of particular fragments from the C-glycoside unit. In order to illustrate some possibilities of these MS techniques, they were applied to the comparative analyses of extracts of Passiflora alata, P. edulis, P. incarnata and P. caerulea (Passifloraceae) that are employed as phytomedicines in Brazil and South America.
使用碰撞诱导解离（CID）的高效液相色谱-质谱联用（HPLC-MS）已被用于鉴定C-糖基黄酮异构体对荭草素/异荭草素和牡荆素/异牡荆素。HPLC-CID/MS分析产生了伪二级质谱图，从而能够鉴定黄酮C-糖苷。通过比较来自C-糖苷单元的特定片段的CID-MS/MS光谱（包括精确质量测量）来实现异构体的有效区分。为了说明这些质谱技术的一些可能性，将它们应用于对西番莲属的具翼西番莲、可食西番莲、 incarnata西番莲和天蓝西番莲（西番莲科）提取物的比较分析，这些植物在巴西和南美洲被用作植物药。
