Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC 88.049-900, Brasil.
Phytochem Anal. 2012 May-Jun;23(3):232-9. doi: 10.1002/pca.1348. Epub 2011 Aug 21.
Leaves and fruits of Passiflora species are widely used around the world in popular medicine, mainly as sedatives and tranquilisers. C-glycosyl flavonoids are the main components of these species.
To investigate the constituent patterns and to develop a chromatographic method for the characterisation of the C-glycosyl flavonoids profile of the extracts of the leaves and the pericarp of South American Passiflora species.
The chemical composition of extracts from the leaves and the fruits' pericarp of Passiflora edulis var. flavicarpa, P. edulis var. edulis, Passiflora alata, Passiflora tripartita var. mollissima, Passiflora quadrangularis, Passiflora manicata and Passiflora ligularis was evaluated for the presence of C-glycosyl flavonoids. Two separate HPLC methods were developed suitable for a diode array detector (DAD) and a MS detector. Separation by HPLC-DAD was achieved on a Luna C-18 column, using solvent A (tetrahydrofuran-isopropanol-acetonitrile) and solvent B (H₃PO₄ 0.5%) in an isocratic elution mode. In the HPLC-MS, the components were separated on a Luna RP-18A column by a gradient elution (water-acetonitrile-formic acid).
The presence of C-glycosyl flavonoids was identified in leaves and pericarp of P. edulis var. flavicarpa, P. alata, P. edulis var. edulis and P. tripartita var. molissima, but only in leaf extracts of P. quadrangularis and P. manicata and not at all in P. ligularis. The different species and varieties showed different major constituents. The C-glycosyl flavonoids identified more frequently were orientin, isoorientin, vitexin and isovitexin.
The methods established are simple and can be used as a tool for the characterisation and quality control of pharmaceutical preparations containing these Passiflora extracts.
西番莲属植物的叶和果实被广泛应用于世界各地的民间医学中，主要作为镇静剂和安定剂。C-糖苷类黄酮是这些物种的主要成分。
研究组成模式，并开发一种色谱方法，用于鉴定南美西番莲属植物叶和果实果皮提取物中 C-糖苷类黄酮的特征。
评估 Passiflora edulis var. flavicarpa、P. edulis var. edulis、Passiflora alata、Passiflora tripartita var. mollissima、Passiflora quadrangularis、Passiflora manicata 和 Passiflora ligularis 的叶和果实果皮提取物中是否存在 C-糖苷类黄酮。开发了两种单独的 HPLC 方法，适用于二极管阵列检测器 (DAD) 和 MS 检测器。HPLC-DAD 分离在 Luna C-18 柱上进行，使用溶剂 A（四氢呋喃-异丙醇-乙腈）和溶剂 B（0.5%H₃PO₄）在等度洗脱模式下进行。在 HPLC-MS 中，在 Luna RP-18A 柱上通过梯度洗脱（水-乙腈-甲酸）分离成分。
在 P. edulis var. flavicarpa、P. alata、P. edulis var. edulis 和 P. tripartita var. molissima 的叶和果皮中鉴定出 C-糖苷类黄酮的存在，但仅在 P. quadrangularis 和 P. manicata 的叶提取物中存在，而在 P. ligularis 中则不存在。不同的物种和品种显示出不同的主要成分。鉴定出的 C-糖苷类黄酮更常见的是Orientin、Isoorientin、Vitexin 和 Isovitexin。
所建立的方法简单，可作为含有这些西番莲属提取物的药物制剂的特征化和质量控制的工具。
