Suljević Enver, Fazlić Muhamed, Corić Jozo, Kiseljaković Jozo Corić i Emina
Institute for Clinical Chemistry and Biochemistry, Clinical Centre of University of Sarajevo.
Bosn J Basic Med Sci. 2003 May;3(2):35-41. doi: 10.17305/bjbms.2003.3553.
Research on the parameters of full blood count and differential white blood count is included in the program of all medical laboratories of primary, secondary and tertiary health care levels. Today, all haematological tests are exclusively performed on the haematology analyzers. Automation of haematology laboratories is a result of the huge requires for haematological test performing, timely issuing of the haematological findings, and possibility of the usage of modern techniques. This work is an evaluation of laser haematology analyzer Cell-Dyn 3700 SL. It investigates the reliability of test results throughout the following parameters: precision, accuracy, sensitivity and specificity of determination methods. It also explores the influence of sample transferring and correlation with haematology analyzer MAXM Retti. Haematology parameters that have been investigated are: white blood cell (WBC), neutrophils (NEU), lymphocytes (LXM), monocytes (MONO), eosinophils (EOS), basophils (BASO), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCHC) red cell distribution width (RDW), platelet (PLT), mean platelet volume (MPV), plateletocrit (PCT), and platelet distribution width (PDW). The results confirms that precision of analyzer fulfils the reproducibility of testing parameters: WBC, RBC, HGB, MCV, MCH, MCHC, and PLT. Correlation coefficient values (r) gained throughout the statistical analysis, that is linear regression results obtained throughout the comparison of two analyzers are adequate except for MCHC (r = 0.64), what is in accordance with literature data. Accuracy is tested by haematology analyzer method and microscopic differentiating method. Correlation coefficient results for granulocytes, lymphocytes and monocytes point the accuracy of methods. Sensitivity and specificity parameters fulfil the analytical criteria. It is confirmed that haematology analyzer Cell-Dyn 3700 SL is reliable for the determination of full blood count in everyday work. Analyzer and its program for differential white blood count can be used for the research and separation of normal and pathological blood counts with addition of microscopic methods confirming distribution or morphologic changes of leukocytes.
全血细胞计数和白细胞分类计数参数的研究包含在初级、二级和三级医疗保健水平的所有医学实验室的项目中。如今，所有血液学检测均在血液分析仪上专门进行。血液学实验室的自动化是由于对血液学检测执行的巨大需求、及时发布血液学检查结果以及使用现代技术的可能性。这项工作是对激光血液分析仪Cell-Dyn 3700 SL的评估。它通过以下参数研究测试结果的可靠性：测定方法的精密度、准确度、灵敏度和特异性。它还探讨了样本转移的影响以及与血液分析仪MAXM Retti的相关性。所研究的血液学参数包括：白细胞（WBC）、中性粒细胞（NEU）、淋巴细胞（LXM）、单核细胞（MONO）、嗜酸性粒细胞（EOS）、嗜碱性粒细胞（BASO）、红细胞（RBC）、血红蛋白（HGB）、血细胞比容（HCT）、平均红细胞体积（MCV）、平均红细胞血红蛋白含量（MCHC）、红细胞分布宽度（RDW）、血小板（PLT）、平均血小板体积（MPV）、血小板比容（PCT）和血小板分布宽度（PDW）。结果证实，分析仪的精密度满足检测参数WBC、RBC、HGB、MCV、MCH、MCHC和PLT的可重复性。通过统计分析获得的相关系数值（r），即在两台分析仪比较中获得的线性回归结果，除MCHC（r = 0.64）外均足够，这与文献数据一致。通过血液分析仪方法和显微镜鉴别方法测试准确度。粒细胞、淋巴细胞和单核细胞的相关系数结果表明了方法的准确性。灵敏度和特异性参数符合分析标准。证实血液分析仪Cell-Dyn 3700 SL在日常工作中用于全血细胞计数的测定是可靠的。分析仪及其白细胞分类计数程序可用于正常和病理性血细胞计数的研究和区分，并辅以显微镜方法确认白细胞的分布或形态变化。
