Singh Poonam, Mishra L C, Pandey Anjali, Iyengar Leela
Biotechnology Laboratory, Department of Chemistry, I.I.T., Kanpur 208 016, India.
Bioresour Technol. 2006 Sep;97(14):1655-9. doi: 10.1016/j.biortech.2005.08.002. Epub 2005 Oct 11.
Studies were carried out on 4-aminobenzenesulfonate (4-ABS) degradation by free and alginate entrapped cells of Agrobacterium sp. PNS-1. Degradation rate in batch reactors with free cells was marginally higher than Ca-encapsulated cells. Comparison of Ca2+ and Ba2+ as gelling agents showed that 4-ABS removal rate was significantly less with Ba-alginate entrapped cells. Specific degradation rates, using linear regression analysis and based on the initial biomass in the beads, varied from 49.7 mg/mg biomass/h to 92.0 mg/mg biomass/h for Ca-alginate encapsulated cells for different initial 4-ABS concentrations ranging from 200 to 800 mg/L. UV spectra of the aliquots drawn at different time intervals from batch reactors did not show accumulation of any intermediate during degradation. Ca-alginate immobilized cells could be repeatedly reused upto five cycles without any loss of activity. Studies with packed bed reactors, operated in a semi-continuous mode, showed that this could be used for 4-ABS degradation.
对农杆菌属PNS-1的游离细胞和海藻酸钠包埋细胞降解4-氨基苯磺酸盐（4-ABS）进行了研究。在装有游离细胞的间歇式反应器中的降解速率略高于钙包埋细胞。作为凝胶剂的Ca2+和Ba2+的比较表明，用海藻酸钡包埋的细胞对4-ABS的去除率显著降低。使用线性回归分析并基于珠子中的初始生物量，对于不同初始4-ABS浓度范围为200至800mg/L的海藻酸钙包埋细胞，比降解速率从49.7mg/mg生物量/小时变化到92.0mg/mg生物量/小时。从间歇式反应器在不同时间间隔取出的等分试样的紫外光谱在降解过程中未显示任何中间产物的积累。海藻酸钙固定化细胞可以重复使用多达五个循环而没有任何活性损失。对半连续运行的填充床反应器的研究表明，这可用于4-ABS的降解。
