Singh Poonam, Birkeland Nils-Kåre, Iyengar Leela, Gurunath Ramanathan
Department of Chemistry, Indian Institute of Technology, 208016, Kanpur, India.
Biodegradation. 2006 Dec;17(6):495-502. doi: 10.1007/s10532-005-9017-7. Epub 2006 Feb 17.
A bacterial strain, PNS-1, isolated from activated sludge, could utilize sulphanilic acid (4-ABS) as the sole organic carbon and energy source under aerobic conditions. Determination and comparison of 16S r DNA sequences showed that the strain PNS-1 is closely related to the species of Agrobacterium genus. Growth on 4-ABS was accompanied with ammonia and sulfate release. TOC results showed complete mineralization of sulphanilic acid. This strain was highly specific for 4-ABS as none of the sulphonated aromatics used in the present study including other ABS isomers were utilized. Strain PNS-1 could, however, utilize all the tested monocyclic aromatic compounds devoid of a sulfonate group. No intermediates could be detected either in the growth phase or with dense cell suspensions. Presence of chloramphenicol completely inhibited 4-ABS degradation by cells pregrown on succinate, indicating that degradation enzymes are inducible. No plasmid could be detected in the Agrobacterium sp. Strain PNS-1 suggesting that 4-ABS degradative genes may be chromosomal encoded.
从活性污泥中分离出的一株细菌PNS-1，在有氧条件下能够利用对氨基苯磺酸（4-ABS）作为唯一的有机碳源和能源。16S r DNA序列的测定和比较表明，菌株PNS-1与土壤杆菌属的物种密切相关。在4-ABS上生长伴随着氨和硫酸盐的释放。TOC结果表明对氨基苯磺酸完全矿化。该菌株对4-ABS具有高度特异性，因为本研究中使用的所有磺化芳烃（包括其他ABS异构体）均未被利用。然而，菌株PNS-1可以利用所有测试的不含磺酸根基团的单环芳香化合物。在生长阶段或浓细胞悬液中均未检测到中间体。氯霉素的存在完全抑制了在琥珀酸盐上预生长的细胞对4-ABS的降解，表明降解酶是可诱导的。在土壤杆菌属菌株PNS-1中未检测到质粒，这表明4-ABS降解基因可能是染色体编码的。
