Nisoli Enzo, Tonello Cristina, Cardile Annalisa, Cozzi Valeria, Bracale Renata, Tedesco Laura, Falcone Sestina, Valerio Alessandra, Cantoni Orazio, Clementi Emilio, Moncada Salvador, Carruba Michele O
Integrated Laboratories Network, Department of Preclinical Sciences, Luigi Sacco Hospital, Milan University, 20157 Milan, Italy.
Science. 2005 Oct 14;310(5746):314-7. doi: 10.1126/science.1117728.
Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3',5'-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals.
热量限制可延长从酵母到哺乳动物等多种生物体的寿命。在此，我们报告，对雄性小鼠进行3个月或12个月的热量限制可诱导内皮型一氧化氮合酶（eNOS）表达以及在其各种组织中形成3',5'-环磷酸鸟苷。这伴随着线粒体生物发生，耗氧量和三磷酸腺苷生成增加，以及沉默调节蛋白1的表达增强。这些效应在eNOS基因敲除小鼠中大大减弱。因此，一氧化氮在热量限制诱导的过程中起基本作用，并且可能参与哺乳动物寿命的延长。
