Zanghi Christine N, Lankes Heather A, Bradel-Tretheway Birgit, Wegman Jessica, Dewhurst Stephen
Department of Microbiology and Immunology, University of Rochester Medical Center, 601 Elmwood Avenue, Box 672, Rochester, NY 14642, USA.
Nucleic Acids Res. 2005 Oct 13;33(18):e160. doi: 10.1093/nar/gni158.
Bacteriophage lambda (lambda) permits the display of many foreign peptides and proteins on the gpD major coat protein. However, some recombinant derivatives of gpD are incompatible with the assembly of stable phage particles. This presents a limitation to current lambda display systems. Here we describe a novel, plasmid-based expression system in which gpD deficient lambda lysogens can be co-complemented with both wild-type and recombinant forms of gpD. This dual expression system permits the generation of mosaic phage particles that contain otherwise recalcitrant recombinant gpD fusion proteins. Overall, this improved gpD display system is expected to permit the expression of a wide variety of peptides and proteins on the surface of bacteriophage lambda and to facilitate the use of modified lambda phage vectors in mammalian gene transfer applications.
λ噬菌体能够在gpD主要衣壳蛋白上展示许多外源肽和蛋白质。然而，gpD的一些重组衍生物与稳定噬菌体颗粒的组装不兼容。这给当前的λ噬菌体展示系统带来了限制。在此，我们描述了一种基于质粒的新型表达系统，其中缺乏gpD的λ溶原菌可以与野生型和重组形式的gpD共同互补。这种双表达系统允许产生包含原本难以处理的重组gpD融合蛋白的嵌合噬菌体颗粒。总体而言，这种改进的gpD展示系统有望在λ噬菌体表面表达多种肽和蛋白质，并有助于在哺乳动物基因转移应用中使用修饰的λ噬菌体载体。
