Minenkova Olga, Pucci Andrea, Pavoni Emiliano, De Tomassi Amedeo, Fortugno Paola, Gargano Nicola, Cianfriglia Maurizio, Barca Stefano, De Placido Sabino, Martignetti Angelo, Felici Franco, Cortese Riccardo, Monaci Paolo
Kenton Labs, c/o Sigma Tau, Pomezia (Roma), Italy.
Laboratorio di Immunologia, Reparto Immunologia dei Tumori, Istituto Superiore di Sanità, Roma, Italy.
Int J Cancer. 2003 Sep 10;106(4):534-544. doi: 10.1002/ijc.11269.
Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage-display technology. We designed a new lambda vector to express protein fragments as N-terminal fusions to the D capsid protein and generated high-complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage-displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients.
用自体患者血清筛选人实体瘤的cDNA文库（SEREX）已被证明是一种识别免疫系统体液免疫所识别的肿瘤抗原的有效方法。在许多情况下，应用这种方法已导致发现作为未知基因产物的新型肿瘤抗原。我们试图通过将SEREX方法与噬菌体展示技术相结合来提高其效力。我们设计了一种新的λ载体，以表达作为D衣壳蛋白N端融合的蛋白片段，并从人乳腺癌细胞系和实体瘤中构建了高复杂性cDNA文库。筛选这些噬菌体展示文库所需的患者血清量有限，并有效地鉴定出了几种与乳腺癌患者血清特异性反应的肿瘤抗原。
