Airs R L, Borrego C M, Garcia-Gil J, Keely B J
Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK,
Photosynth Res. 2001;70(2):221-30. doi: 10.1023/A:1015146304441.
Detailed APCI LC-MS/MS analysis using an improved HPLC separation reveals the green sulphur bacterium Chlorobium phaeobacteroides strain UdG6053 to contain a wider range of distinct bacteriochlorophyll homologues than has been previously recognised in Chlorobiaceae. The diversity in the homologue distribution is confirmed as arising from differences in the extent of alkylation of the macrocycle and variation in the nature of the esterifying alcohol and a novel series of bacteriochlorophyll structures has been recognised. Homologues containing esterifying alcohols other than farnesol, a number of which have not previously been reported in Chlorobiaceae, are present in high relative abundance. Confirmation of the structures of the esterifying alcohols has been obtained by hydrolysis and analysis by GC-MS.
使用改进的高效液相色谱分离进行的详细大气压化学电离液相色谱-串联质谱分析表明，绿色硫细菌褐黄绿菌UdG6053菌株所含的不同细菌叶绿素同系物范围比之前在绿菌科中所认识到的更广。同系物分布的多样性被确认为源于大环烷基化程度的差异以及酯化醇性质的变化，并且已经识别出一系列新的细菌叶绿素结构。含有法尼醇以外酯化醇的同系物以高相对丰度存在，其中一些在绿菌科中以前尚未有报道。通过水解和气相色谱-质谱分析已获得酯化醇结构的确证。
