Bassham James A
, 785 Balra Drive, El Cerrito, CA, 94530-3302, USA,
Photosynth Res. 2003;76(1-3):35-52. doi: 10.1023/A:1024929725022.
The photosynthetic carbon reduction cycle was elucidated through the use of (14)CO(2) during photosynthesis to label metabolic intermediates. Mapping and proof of the cycle required identification of labeled metabolites, observation of changes in levels of labeled metabolites during transitions from light to dark and from high to low CO(2) levels, determination of intramolecular distribution of (14)C within the metabolites after a few seconds of photosynthesis with (14)CO(2), and estimation of metabolite concentrations, used to calculate true free energy changes at each step in the cycle.
光合碳还原循环是通过在光合作用过程中使用（14）CO₂标记代谢中间体来阐明的。该循环的绘制和验证需要鉴定标记的代谢物，观察从光照到黑暗以及从高CO₂水平到低CO₂水平转变过程中标记代谢物水平的变化，在使用（14）CO₂进行几秒钟光合作用后测定代谢物分子内（14）C的分布，以及估算代谢物浓度，用于计算循环中每个步骤的真实自由能变化。
