Wei Qin-Zhi, Zhuang Zhi-Xiong, Yuan Jian-Hui, Huang Hai-Yan, Li Xiyi, Yang Xiaohua, Geng Lei
Department of Health Toxicology, Sun Yat-sen Medical University, Guangzhou 510080, China.
Wei Sheng Yan Jiu. 2005 Jul;34(4):403-6.
To observe the differentially expressed genes of the human normal liver cell (L-02) induced by low concentration of trichloroethylene (TCE).
The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes.
The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.
观察低浓度三氯乙烯（TCE）诱导人正常肝细胞（L-02）的差异表达基因。
采用MTT法分析TCE毒性的剂量效应关系。选择5 μmol/L作为低浓度，40 μmol/L作为高浓度。然后分别用低浓度、高浓度TCE处理L-02细胞，以及先用低浓度TCE预处理后再用高浓度TCE攻击L-02细胞。应用荧光差异显示聚合酶链反应（Fluoro DD-PCR）技术寻找TCE不同处理组的差异表达基因。结果共发现51条差异表达条带，对其中11条差异条带进行了克隆和测序。其中9条为已知基因，2条为新基因。
对差异条带进行鉴定，可为深入研究低浓度TCE诱导L-02细胞适应性反应的机制提供科学依据。
