Koskinen Walter J, Bono Petri, Leivo Ilmo, Vaheri Antti, Aaltonen Leena-Maija, Joensuu Heikki
Department of Virology, Haartman Institute, University of Helsinki, Finland.
Radiother Oncol. 2005 Nov;77(2):172-5. doi: 10.1016/j.radonc.2005.09.013. Epub 2005 Oct 17.
Early stage vocal cord carcinomas are usually cured by radiation therapy despite the use of portals that exclude the cervical lymph nodes. We investigated whether the lymphatic vessel density (LVD) of vocal cord carcinomas differs from that of other head and neck carcinomas.
Deparaffinized tissue from tumors of 60 patients diagnosed with head and neck squamous cell carcinoma (HNSCC) were immunostained for LYVE-1, a novel lymphatic vessel marker. Twenty-two had vocal cord carcinoma. Tumor blood vessel density (BVD) was assessed using immunostaining for CD31.
Tumor overall LVD, including both intra- and peritumoral lymph vessels, was 10-fold lower than the BVD (5 counts/mm2 vs. 52 mm(-2), respectively). A high LVD was associated with a high BVD (P = .002), but neither was associated with the tumor size. Both tumor LVD and BVD were lower in vocal cord carcinomas than in HNSCCs arising at other sites (median, 0 vs. 7 mm(-2), P=.016; and median, 36 vs. 52 mm(-2), P = .006, respectively). Only one vocal cord carcinoma was associated with a regional metastasis at the time of the diagnosis. Among the rest of the cases tumor size was a better predictor for the presence of regional metastases than tumor BVD or LVD in a logistic regression model (odds ratio 2.2, 95% CI 1.1-4.5).
Vocal cord carcinomas have a low lymph vessel density as compared with HNSCCs arising at other sites.
早期声带癌通常通过放射治疗治愈，尽管放疗野未包括颈部淋巴结。我们研究了声带癌的淋巴管密度（LVD）是否与其他头颈部癌不同。
对60例诊断为头颈部鳞状细胞癌（HNSCC）患者的肿瘤石蜡包埋组织进行免疫染色，检测新型淋巴管标志物LYVE-1。其中22例为声带癌。采用免疫染色检测CD31评估肿瘤血管密度（BVD）。
肿瘤总体LVD，包括瘤内和瘤周淋巴管，比BVD低10倍（分别为5个/mm²对52个/mm⁻²）。高LVD与高BVD相关（P = .002），但两者均与肿瘤大小无关。声带癌的肿瘤LVD和BVD均低于其他部位发生的HNSCC（中位数分别为0对7个/mm⁻²，P = .016；以及中位数36对52个/mm⁻²，P = .006）。仅1例声带癌在诊断时伴有区域转移。在其余病例中，在逻辑回归模型中，肿瘤大小比肿瘤BVD或LVD更能预测区域转移的存在（优势比2.2，95%可信区间1.1 - 4.5）。
与其他部位发生的HNSCC相比，声带癌的淋巴管密度较低。
