Czéh G, Aitken P G, Somjen G G
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.
Neuroreport. 1992 Feb;3(2):197-200. doi: 10.1097/00001756-199202000-00019.
In rat hippocampal tissue slices we recorded extracellular potential (Vo) and whole-cell patch clamp current of CA1 pyramid cells. During hypoxic spreading depression (SD)-like depolarization, the holding current (Ih) increased sharply. Membrane 'slope' resistance (Rm) decreased to 10-67% (mean 39%) of the resting value. The SD-related membrane current (ISD) reversed near zero mV. With voltage dependent K+ and Na+ currents blocked by Cs+ and QX-314, shifts of Ih and decrease of Rm during SD were not suppressed. We conclude that hypoxic SD of CA1 pyramidal cells is associated with a large non-selective inward current through yet to be identified membrane mechanisms, which cannot fully explain the SD-related Vo shift.
在大鼠海马组织切片中，我们记录了CA1锥体神经元的细胞外电位（Vo）和全细胞膜片钳电流。在类似缺氧性扩散性抑制（SD）的去极化过程中，钳制电流（Ih）急剧增加。膜“斜率”电阻（Rm）降至静息值的10 - 67%（平均39%）。与SD相关的膜电流（ISD）在接近0 mV时反转。当电压依赖性K⁺和Na⁺电流被Cs⁺和QX - 314阻断时，SD期间Ih的变化和Rm的降低并未受到抑制。我们得出结论，CA1锥体神经元的缺氧性SD与一种通过尚未明确的膜机制产生的大量非选择性内向电流有关，而这并不能完全解释与SD相关的Vo变化。
