Tanji Yasunori, Mizoguchi Katsunori, Yoichi Masatoshi, Morita Masatomo, Hori Katsutoshi, Unno Hajime
Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuts-cho, Midori-ku, Yokohama 226-8501, Japan.
J Biosci Bioeng. 2002;94(2):172-4. doi: 10.1263/jbb.94.172.
The fate of coliphage in a wastewater treatment plant in the central part of Japan was investigated from March to December 2001. A relative abundance of coliphage, 1000-10,000 PFU/ml determined with three different Escherichia coli strains, was detected in the influent. But, no remarkable seasonal change in the phage concentration in the influent was observed during the ten-month test period. Almost ten times higher coliphage concentration was detected by the F+ E. coli strain than by the other two F- strains. The RNA phage was more stable than the DNA phage against aerobic treatment using activated sludge. Most of the phages in the influent and primary settling tank were detected as suspended forms. Anaerobic-aerobic treatment enhanced adsorption of the phage by the solid particles. Almost no phage was detected in the effluent. Aerobic treatment using activated sludge and/or the addition of flocculants such as PAC was effective for the removal of coliphage, an index of enteric viral pollution.
2001年3月至12月，对日本中部一家污水处理厂中大肠杆菌噬菌体的命运进行了调查。在进水口检测到相对丰度的大肠杆菌噬菌体，用三种不同的大肠杆菌菌株测定其浓度为1000 - 10000 PFU/ml。但是，在为期十个月的测试期内，未观察到进水口中噬菌体浓度有明显的季节性变化。F+大肠杆菌菌株检测到的噬菌体浓度几乎比其他两种F - 菌株高十倍。RNA噬菌体比DNA噬菌体对活性污泥的好氧处理更稳定。进水口和初沉池中的大多数噬菌体以悬浮形式被检测到。厌氧 - 好氧处理增强了噬菌体被固体颗粒的吸附。在出水口几乎未检测到噬菌体。使用活性污泥进行好氧处理和/或添加絮凝剂（如PAC）对去除作为肠道病毒污染指标的大肠杆菌噬菌体是有效的。
