Department of Environmental Sciences, Directorate of Natural Resources Management, Tamil Nadu Agricultural University, 641003, Tamil Nadu, India.
J Environ Health Sci Eng. 2013 Jun 28;11(1):12. doi: 10.1186/2052-336X-11-12.
Many sewage waste treatment systems are aiming for complete pathogen removal which necessitates search for novel approaches that does not harm the environment. One such novel approach is exploring the possibilities of bacteriophages for pathogen removal. Hospital wastewater was collected from different locations of Tamil Nadu and used for the study. The total heterotroph and total coliform population ranged from 1.6 × 105 to 8.3 × 106 per mL and from 1.2 × 103 to 1.6 × 103/ 100 mL of sample respectively. Higher frequency of antibiotic resistant E. coli, Pseudomonas sp. Streptococcus sp and Bacillus spp were observed in all the places, which clearly indicated the extent of pollution. All the samples had specific phages against E. coli and none of the samples had phages against MTCC culture. E. coli specific phage was isolated and the population of phage required for effective killing of E. coli has been standardized as 3 × 104 pfu / mL of lysate. The inoculation resulted in 100% removal of pathogen from sewage water within 14 hours of incubation.
许多污水废物处理系统都致力于彻底去除病原体，这就需要寻找新的方法，而这些新方法不应损害环境。一种新方法是探索噬菌体在去除病原体方面的可能性。从泰米尔纳德邦的不同地点收集医院废水用于本研究。总异养菌和总大肠菌群的数量分别为每毫升 1.6×105 至 8.3×106 个和每 100 毫升样品 1.2×103 至 1.6×103 个。在所有地方都观察到更高频率的抗生素耐药大肠杆菌、假单胞菌、链球菌和芽孢杆菌，这清楚地表明了污染的程度。所有样本都针对大肠杆菌具有特定的噬菌体，而没有样本针对 MTCC 培养物具有噬菌体。分离出了针对大肠杆菌的噬菌体，并将有效杀死大肠杆菌所需的噬菌体数量标准化为裂解物中的 3×104 pfu/mL。接种后，在 14 小时的孵育时间内，污水中的病原体被 100%去除。
