寡核苷酸工厂：一种用于交互式寡核苷酸设计的可视化工具。

OligoFaktory: a visual tool for interactive oligonucleotide design.

作者信息

Schretter Colas, Milinkovitch Michel C

机构信息

Unit of Evolutionary Genetics, Institute of Molecular Biology and Medicine, Free University of Brussels, Gosselies, Belgium.

出版信息

Bioinformatics. 2006 Jan 1;22(1):115-6. doi: 10.1093/bioinformatics/bti728. Epub 2005 Oct 20.

DOI:10.1093/bioinformatics/bti728

PMID:16239305

Abstract

SUMMARY

The OligoFaktory is a set of tools for the design, on an arbitrary number of target sequences, of high-quality long oligonucleotide for micro-array, of primer pair for PCR, of siRNA and more. The user-centered interface exists in two flavours: a web portal and a standalone software for Mac OS X Tiger. A unified presentation of results provides overviews with distribution charts and relative location bar graphs, as well as detailed features for each oligonucleotide. Input and output files conform to a common XML interchange file format to allow both automatic generation of input data, archiving, and post-processing of results. The design pipeline can use BLAST servers to evaluate specificity of selected oligonucleotides.

AVAILABILITY

The web portal http://ueg.ulb.ac.be/oligofaktory/; the software for Macintosh: http://www.oligofaktory.org/

摘要

寡核苷酸工厂（OligoFaktory）是一套工具，可针对任意数量的靶序列设计用于微阵列的高质量长寡核苷酸、用于聚合酶链式反应（PCR）的引物对、小干扰RNA（siRNA）等。以用户为中心的界面有两种形式：一个门户网站和一个适用于Mac OS X Tiger的独立软件。结果的统一呈现提供了带有分布图和相对位置柱状图的概述，以及每个寡核苷酸的详细特征。输入和输出文件符合通用的XML交换文件格式，以允许自动生成输入数据、存档和结果的后处理。设计流程可以使用BLAST服务器来评估所选寡核苷酸的特异性。