Kline L W, Kaneko T, Chiu K W, Pang P K
Department of Oral Biology, University of Alberta, Edmonton, Canada.
Gen Comp Endocrinol. 1992 Jul;87(1):14-9. doi: 10.1016/0016-6480(92)90144-9.
The presence of a calcitonin gene-related peptide (CGRP)-like material was demonstrated in the gallbladder of the bullfrog, Rana catesbeiana, using immunocytochemistry and confirmed by radioimmunoassay. An intense immunocytochemical reaction was observed in nerves located in the smooth muscle layers and associated with blood vessels. No immunoreactive nerve fibers were associated with ganglia, nor were immunoreactive cell bodies observed. Radioimmunoassay showed that 25.03 +/- 2.5 pmol/g tissue of CGRP-like material was present. In vitro tension studies using gallbladder strips showed that CGRP exerted an inhibitory effect on both acetylcholine- and cholecystokinin octapeptide-induced tension but had no effect on KCl-, norepinephrine-, or cerulein-induced tension. CGRP may act directly on the gallbladder smooth muscle to inhibit contraction.
运用免疫细胞化学方法在牛蛙（北美牛蛙）的胆囊中证实了降钙素基因相关肽（CGRP）样物质的存在，并通过放射免疫测定法加以确认。在位于平滑肌层且与血管相关的神经中观察到强烈的免疫细胞化学反应。未发现与神经节相关的免疫反应性神经纤维，也未观察到免疫反应性细胞体。放射免疫测定显示，存在25.03±2.5 pmol/g组织的CGRP样物质。使用胆囊条进行的体外张力研究表明，CGRP对乙酰胆碱和八肽胆囊收缩素诱导的张力均有抑制作用，但对氯化钾、去甲肾上腺素或蛙皮素诱导的张力无影响。CGRP可能直接作用于胆囊平滑肌以抑制收缩。
