Kline L W, Kaneko T, Benishin C G, Pang P K
Department of Physiology, University of Alberta, Edmonton, Canada.
Can J Physiol Pharmacol. 1991 Aug;69(8):1149-54. doi: 10.1139/y91-168.
Calcitonin gene-related peptide (CGRP) relaxes vascular and intestinal smooth muscle. This study localized CGRP in the guinea pig gallbladder, examined the effects of CGRP on KCl- and ACh-induced contraction, and determined CGRPs site of action in the gallbladder. The gallbladder of male Hartley guinea pigs was used in in vitro tension studies, radioimmunoassay, or immunocytochemical studies. Radioimmunoassay showed that 8.0 +/- 0.5 pmol/g of immunoreactive CGRP was present. Immunocytochemistry demonstrated that immunoreactive-CGRP nerve fibers occurred around blood vessels, in gallbladder smooth muscle layers, and were associated with ganglia. No immunoreactive cell bodies were observed, even after colchicine treatment. The in vitro tension studies showed that CGRP inhibits either KCl- or acetylcholine-stimulated contraction. CGRP may in part act directly on the gallbladder smooth muscle to inhibit contraction.
降钙素基因相关肽（CGRP）可舒张血管和平滑肌。本研究对豚鼠胆囊中的CGRP进行定位，检测CGRP对氯化钾和乙酰胆碱诱导收缩的影响，并确定CGRP在胆囊中的作用位点。雄性Hartley豚鼠的胆囊用于体外张力研究、放射免疫分析或免疫细胞化学研究。放射免疫分析显示，存在8.0±0.5 pmol/g的免疫反应性CGRP。免疫细胞化学表明，免疫反应性CGRP神经纤维出现在血管周围、胆囊平滑肌层，并与神经节相关。即使经过秋水仙碱处理，也未观察到免疫反应性细胞体。体外张力研究表明，CGRP可抑制氯化钾或乙酰胆碱刺激的收缩。CGRP可能部分直接作用于胆囊平滑肌以抑制收缩。
