Shimada Yasushi, Seki Yuichi, Uzzaman Mohammed Akhtar, Sattabanasuk Vanthana, Sasafuchi Yasutaka, Foxton Richard M, Otsuki Masayuki, Tagami Junji
Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
J Adhes Dent. 2005 Autumn;7(3):247-51.
The objective of this study was to evaluate the pulpal response to a newly-developed MMA resin cement (MultiBond, Tokuyama) when used for adhesively luting composite resin inlays.
Cervical cavities were prepared in monkey teeth. The teeth were randomly divided into 3 groups. In the experimental group, a self-etching primer and a resin cement were applied to the cavities, and then hybrid composite inlays (Estenia, Kuraray) were inserted using freshly mixed resin cement. In the other groups, a zinc oxide/eugenol cement (Eugedain, Showa Yakuhin Kakou) or a glass-ionomer cement (Fuji II, GC) was used to fill the cavity. The teeth were then extracted after 3, 30, and 90 days, fixed in 10% buffered formalin solution, and prepared using routine histological techniques. Five-mum-thick sections were stained with hematoxylin and eosin, or Brown & Brenn gram stain for bacterial observation. Histopathological reactions in the pulp tissue and bacterial penetration along the cavity walls were assessed using a standardized score.
No serious inflammatory reactions in the pulp, such as necrosis or abscess formation, were observed in any of the experimental periods, except for 1 case after 30 days, in which a pulpal exposure was suspected. Disarrangement of the odontoblast layer and deposition of reparative dentin were the major reactions observed in this specimen. No bacterial penetration along the cavity walls was detected. The monkey pulpal response and in vivo sealing ability of the MMA resin cement in combination with the self-etching primer was considered as good as that of the glass-ionomer cement.
The new MMA resin cement showed acceptable biological compatibility to the monkey pulp when used to adhesively lute composite resin inlays.
本研究的目的是评估一种新开发的甲基丙烯酸甲酯（MMA）树脂水门汀（MultiBond，德山）用于粘结复合树脂嵌体时的牙髓反应。
在猴牙上制备颈部洞形。将牙齿随机分为3组。实验组中，在洞形内应用自酸蚀底漆和树脂水门汀，然后使用新鲜混合的树脂水门汀插入混合复合树脂嵌体（Estenia，可乐丽）。在其他组中，使用氧化锌/丁香酚水门汀（Eugedain，昭和药业）或玻璃离子水门汀（Fuji II，GC）填充洞形。然后在3天、30天和90天后拔牙，固定于10%缓冲甲醛溶液中，并采用常规组织学技术制备标本。5μm厚的切片用苏木精和伊红染色，或用Brown & Brenn革兰氏染色观察细菌。使用标准化评分评估牙髓组织中的组织病理学反应以及沿洞壁的细菌侵入情况。
在任何实验时间段内，除30天后有1例疑似牙髓暴露外，均未观察到牙髓有严重的炎症反应，如坏死或脓肿形成。该标本中观察到的主要反应为成牙本质细胞层排列紊乱和修复性牙本质沉积。未检测到沿洞壁的细菌侵入。MMA树脂水门汀与自酸蚀底漆联合使用时，猴牙髓反应和体内封闭能力被认为与玻璃离子水门汀相当。
当新的MMA树脂水门汀用于粘结复合树脂嵌体时，对猴牙髓显示出可接受的生物相容性。
