用于粪便脂肪测量的近红外光谱法：与传统重量法和滴定法的比较。
Near infrared spectrometry for faecal fat measurement: comparison with conventional gravimetric and titrimetric methods.
作者信息
Benini L, Caliari S, Guidi G C, Vaona B, Talamini G, Vantini I, Scuro L A
机构信息
Instituto di Clinica Medica, University of Verona, Italy.
出版信息
Gut. 1989 Oct;30(10):1344-7. doi: 10.1136/gut.30.10.1344.
This investigation was aimed at comparing a new method for measuring faecal fat excretion, carried out with a semi-automated instrument by using near infrared analysis (NIRA), with the traditional titrimetric (Van de Kamer) and gravimetric (Sobel) methods. Near infrared analysis faecal fat was assayed on the three day stool collection from 118 patients (68 chronic pancreatitis, 19 organic diseases of the gastrointestinal tract, 19 alcoholic liver disease, 12 functional gastrointestinal disorders). A strict linear correlation was found between NIRA and both the titrimetric (r = 0.928, p less than 0.0001) and the gravimetric (r = 0.971, p less than 0.0001) methods. On homogenised faeces, a mean coefficient of variation of 2.1 (SD 1.71)% was found. Before homogenisation (where a mean coefficient of variation of 7% was found) accurate results were obtained when the mean of five measurements was considered. In conclusion, the assay of faecal fat excretion by the near infrared reflessometry appears a simple, rapid and reliable method for measuring steatorrhoea.
本研究旨在比较一种采用近红外分析（NIRA）的半自动仪器测量粪便脂肪排泄的新方法与传统的滴定法（范德卡默法）和重量法（索贝尔法）。对118例患者（68例慢性胰腺炎、19例胃肠道器质性疾病、19例酒精性肝病、12例功能性胃肠疾病）连续三天收集的粪便进行近红外分析粪便脂肪检测。结果发现，NIRA与滴定法（r = 0.928，p < 0.0001）和重量法（r = 0.971，p < 0.0001）均存在严格的线性相关性。对于匀浆粪便，变异系数平均值为2.1（标准差1.71）%。在匀浆前（变异系数平均值为7%），当考虑五次测量的平均值时可获得准确结果。总之，近红外反射光度法检测粪便脂肪排泄似乎是一种测量脂肪泻的简单、快速且可靠的方法。
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