Kitamura A, Yamashita Y, Hasegawa Y, Kojima H, Nagasawa T, Mori N
Department of Pathology of Biological Response, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Histopathology. 2005 Nov;47(5):523-32. doi: 10.1111/j.1365-2559.2005.02265.x.
Most lymphomas arising in the nasal cavity are thought to be of natural killer (NK) cell origin. However, some reports indicate that T- and B-cell lymphomas may also primarily arise in the nasal cavity. We therefore studied lymphomas arising in the nasal cavity both histologically and immunohistochemically.
Of the 32 cases investigated, 20 cases were also available as fresh frozen specimens. We diagnosed 31 cases as extranodal NK/T-cell lymphoma and one as plasmacytoma. The neoplastic cells were immunoreactive for CD3 (polyclonal) 31/31, LMP-1 12/31, CD20 (L26) 0/31, granzyme B 30/31, TIA-1 30/30, CD56 (123C3) 29/31, CD4 0/31 and CD8 3/31. In situ hybridization for Epstein-Barr virus-encoded small RNA-1 (EBER-1) was detected in 31/31. In frozen tissue sections, neoplastic cells mostly showed CD3 (Leu4)-, CD4 (Leu3a)-, CD5 (Leu1)-, CD8 (Leu2)-, CD16 (Leu11)-, CD56 (Leu19)+, betaF1-, TCRdelta1-, perforin+, CD94+ phenotypes. These immunohistochemical findings indicate their NK cell origin. In three cases, neoplastic cells were positive for CD8. In one of these cases, neoplastic cells were positive for CD8beta and Valpha24, suggesting their NKT-cell origin.
Our present study indicates that primary lymphomas arising in the nasal cavity are mostly of NK cell derivation. Our present study also suggests that a small number of cases are derived from NKT-cells.
大多数起源于鼻腔的淋巴瘤被认为是自然杀伤（NK）细胞来源。然而，一些报告表明T细胞和B细胞淋巴瘤也可能主要起源于鼻腔。因此，我们对起源于鼻腔的淋巴瘤进行了组织学和免疫组织化学研究。
在研究的32例病例中，20例也有新鲜冰冻标本。我们诊断出31例为结外NK/T细胞淋巴瘤，1例为浆细胞瘤。肿瘤细胞对CD3（多克隆）呈免疫反应阳性（31/31），LMP-1为12/31，CD20（L26）为0/31，颗粒酶B为30/31，TIA-1为30/30，CD56（123C3）为29/31，CD4为0/31，CD8为3/31。31/31检测到爱泼斯坦-巴尔病毒编码的小RNA-1（EBER-1）原位杂交阳性。在冰冻组织切片中，肿瘤细胞大多表现为CD3（Leu4）-、CD4（Leu3a）-、CD5（Leu1）-、CD8（Leu2）-、CD16（Leu11）-、CD56（Leu19）+、betaF1-、TCRdelta1-、穿孔素+、CD94+表型。这些免疫组织化学结果表明其NK细胞来源。在3例中，肿瘤细胞CD8呈阳性。其中1例肿瘤细胞CD8β和Valpha24呈阳性，提示其NKT细胞来源。
我们目前的研究表明，起源于鼻腔的原发性淋巴瘤大多来源于NK细胞。我们目前的研究还表明，少数病例来源于NKT细胞。
