• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

一种改良的黏附试验与现有用于鉴定肠集聚性大肠杆菌的试验方法的比较。

Comparison of a modified adherence assay with existing assay methods for identification of enteroaggregative Escherichia coli.

作者信息

Haider K, Faruque S M, Albert M J, Nahar S, Neogi P K, Hossain A

机构信息

International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.

出版信息

J Clin Microbiol. 1992 Jun;30(6):1614-6. doi: 10.1128/jcm.30.6.1614-1616.1992.

DOI:10.1128/jcm.30.6.1614-1616.1992
PMID:1624587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265346/
Abstract

Localized, diffuse, and aggregative adherence patterns of Escherichia coli identified with specific DNA probes were compared in cell culture adherence assays by using the Center for Vaccine Development, Baltimore, method, the University of Texas Medical School, Houston (UTH), method, and a modified UTH method. Increasing postwash incubation time from 2 to 4 h in the modified UTH method allowed identification of enteroaggregative E. coli, which was otherwise not identified by the original UTH method.

摘要

在细胞培养黏附试验中,采用巴尔的摩疫苗开发中心的方法、休斯顿德克萨斯大学医学院(UTH)的方法以及改良的UTH方法,比较了用特异性DNA探针鉴定的大肠杆菌的局部、弥漫和聚集黏附模式。在改良的UTH方法中,将洗涤后孵育时间从2小时延长至4小时,能够鉴定出肠聚集性大肠杆菌,而原始的UTH方法无法鉴定出这种细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1285/265346/225541ba6cd3/jcm00030-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1285/265346/225541ba6cd3/jcm00030-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1285/265346/225541ba6cd3/jcm00030-0269-a.jpg

相似文献

1
Comparison of a modified adherence assay with existing assay methods for identification of enteroaggregative Escherichia coli.一种改良的黏附试验与现有用于鉴定肠集聚性大肠杆菌的试验方法的比较。
J Clin Microbiol. 1992 Jun;30(6):1614-6. doi: 10.1128/jcm.30.6.1614-1616.1992.
2
Comparison of two assay methods for patterns of adherence to HEp-2 cells of Escherichia coli from patients with diarrhea.两种检测腹泻患者大肠杆菌对HEp-2细胞黏附模式的方法比较。
J Clin Microbiol. 1990 May;28(5):882-5. doi: 10.1128/jcm.28.5.882-885.1990.
3
Adherence patterns and adherence-related DNA sequences in Escherichia coli isolates from children with and without diarrhea in São Paulo city, Brazil.巴西圣保罗市腹泻及非腹泻儿童分离出的大肠杆菌中的黏附模式及黏附相关DNA序列
J Clin Microbiol. 1998 Dec;36(12):3609-13. doi: 10.1128/JCM.36.12.3609-3613.1998.
4
[PCR methods for detection of enteropathogenic Escherichia coli (localized adherence) and enteroaggregative Escherichia coli].
Kansenshogaku Zasshi. 1996 Jun;70(6):569-73. doi: 10.11150/kansenshogakuzasshi1970.70.569.
5
Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells.致泻性大肠杆菌对HEp-2细胞的黏附模式。
Pediatr Infect Dis J. 1987 Sep;6(9):829-31. doi: 10.1097/00006454-198709000-00008.
6
Localized, diffuse, and aggregative-adhering Escherichia coli from infants with acute diarrhea and matched-controls.来自急性腹泻婴儿及配对对照的局部性、弥漫性和聚集黏附性大肠杆菌
Mem Inst Oswaldo Cruz. 1992 Jan-Mar;87(1):93-7. doi: 10.1590/s0074-02761992000100015.
7
Diarrhoeal disease: current concepts and future challenges. Enteroadherent Escherichia coli: a heterogeneous group of E. coli implicated as diarrhoeal pathogens.腹泻病:当前概念与未来挑战。肠道黏附性大肠杆菌:一类被认为是腹泻病原体的异质性大肠杆菌群。
Trans R Soc Trop Med Hyg. 1993 Dec;87 Suppl 3:49-53. doi: 10.1016/0035-9203(93)90539-3.
8
Localized, aggregative, and diffuse adherence to HeLa cells, plastic, and human small intestines by Escherichia coli isolated from patients with diarrhea.从腹泻患者中分离出的大肠杆菌对HeLa细胞、塑料和人类小肠的局部、聚集性和弥漫性黏附
J Infect Dis. 1992 Dec;166(6):1295-310. doi: 10.1093/infdis/166.6.1295.
9
Phenotypic and genetic analysis of diarrhea-associated Escherichia coli isolated from children in the United Kingdom.
J Pediatr Gastroenterol Nutr. 2001 Jul;33(1):32-40. doi: 10.1097/00005176-200107000-00006.
10
[Association of patterns of Escherichia coli adherence to HEp-2 cells with acute and persistent diarrhea].[大肠杆菌对HEp-2细胞的黏附模式与急性和持续性腹泻的关联]
Arq Gastroenterol. 1999 Jan-Mar;36(1):54-60.

引用本文的文献

1
Age-specific prevalence of Escherichia coli with localized and aggregative adherence in Venezuelan infants with acute diarrhea.委内瑞拉急性腹泻婴儿中具有局部和聚集性黏附特性的大肠杆菌的年龄特异性患病率。
J Clin Microbiol. 1997 May;35(5):1103-7. doi: 10.1128/jcm.35.5.1103-1107.1997.
2
Enteroadherent Escherichia coli and diarrhea in children: a prospective case-control study.肠道黏附性大肠杆菌与儿童腹泻:一项前瞻性病例对照研究。
J Clin Microbiol. 1996 Dec;34(12):2897-903. doi: 10.1128/jcm.34.12.2897-2903.1996.
3
Adhesion and its role in the virulence of enteropathogenic Escherichia coli.

本文引用的文献

1
Surveillance of patients attending a diarrhoeal disease hospital in Bangladesh.对孟加拉国一家腹泻病医院就诊患者的监测。
Br Med J (Clin Res Ed). 1982 Oct 23;285(6349):1185-8. doi: 10.1136/bmj.285.6349.1185.
2
Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.肠致病性大肠杆菌对HeLa细胞的独特黏附模式。
Infect Immun. 1984 Aug;45(2):534-6. doi: 10.1128/iai.45.2.534-536.1984.
3
Pathogenicity of enteroadherent Escherichia coli in adult volunteers.成人志愿者中肠黏附性大肠杆菌的致病性
黏附及其在肠致病性大肠杆菌毒力中的作用。
Clin Microbiol Rev. 1994 Apr;7(2):152-73. doi: 10.1128/CMR.7.2.152.
4
Escherichia coli adherence to HEp-2 cells with prefixed cells.用固定细胞检测大肠杆菌对HEp-2细胞的黏附
J Clin Microbiol. 1995 May;33(5):1414-7. doi: 10.1128/jcm.33.5.1414-1417.1995.
J Infect Dis. 1986 Sep;154(3):524-7. doi: 10.1093/infdis/154.3.524.
4
Prospective study of diarrhoeal disease in a cohort of rural Mexican children: incidence and isolated pathogens during the first two years of life.墨西哥农村儿童队列中腹泻病的前瞻性研究:生命最初两年的发病率及分离出的病原体
Epidemiol Infect. 1988 Aug;101(1):123-34. doi: 10.1017/s0950268800029289.
5
Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells.致泻性大肠杆菌对HEp-2细胞的黏附模式。
Pediatr Infect Dis J. 1987 Sep;6(9):829-31. doi: 10.1097/00006454-198709000-00008.
6
Enteroadherent Escherichia coli as a cause of diarrhea among children in Mexico.肠粘附性大肠杆菌是墨西哥儿童腹泻的一个病因。
J Clin Microbiol. 1987 Oct;25(10):1917-9. doi: 10.1128/jcm.25.10.1917-1919.1987.
7
Use of DNA probes and HEp-2 cell adherence assay to detect diarrheagenic Escherichia coli.使用DNA探针和HEp-2细胞粘附试验检测致泻性大肠杆菌。
J Infect Dis. 1988 Jul;158(1):224-8. doi: 10.1093/infdis/158.1.224.
8
Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease.肠黏附-聚集性大肠杆菌的特性研究,腹泻病的一种假定病原体
J Infect Dis. 1988 Jul;158(1):70-9. doi: 10.1093/infdis/158.1.70.
9
Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India.印度一组农村儿童中与持续性腹泻相关的聚集性大肠杆菌
J Infect Dis. 1989 Jun;159(6):1061-4. doi: 10.1093/infdis/159.6.1061.
10
Comparison of two assay methods for patterns of adherence to HEp-2 cells of Escherichia coli from patients with diarrhea.两种检测腹泻患者大肠杆菌对HEp-2细胞黏附模式的方法比较。
J Clin Microbiol. 1990 May;28(5):882-5. doi: 10.1128/jcm.28.5.882-885.1990.