用固定细胞检测大肠杆菌对HEp-2细胞的黏附
Escherichia coli adherence to HEp-2 cells with prefixed cells.
作者信息
Zepeda-Lopez H M, Gonzalez-Lugo G M
机构信息
Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Mexico D.F., Mexico.
出版信息
J Clin Microbiol. 1995 May;33(5):1414-7. doi: 10.1128/jcm.33.5.1414-1417.1995.
Abstract
We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live cells.
摘要
我们描述了一种新方法，该方法使用冷的无水甲醇预处理细胞，以使肠致病性大肠杆菌黏附于HEP-2细胞。我们发现，一种将细菌在检测肉汤中培养至10⁶至10⁷CFU/ml，并与预处理细胞在37℃下孵育3小时的方法，相对于使用活细胞的方法，显示出100%的敏感性和特异性。
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