• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用固定细胞检测大肠杆菌对HEp-2细胞的黏附

Escherichia coli adherence to HEp-2 cells with prefixed cells.

作者信息

Zepeda-Lopez H M, Gonzalez-Lugo G M

机构信息

Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Mexico D.F., Mexico.

出版信息

J Clin Microbiol. 1995 May;33(5):1414-7. doi: 10.1128/jcm.33.5.1414-1417.1995.

DOI:10.1128/jcm.33.5.1414-1417.1995
PMID:7615770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228182/
Abstract

We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live cells.

摘要

我们描述了一种新方法，该方法使用冷的无水甲醇预处理细胞，以使肠致病性大肠杆菌黏附于HEP-2细胞。我们发现，一种将细菌在检测肉汤中培养至10⁶至10⁷CFU/ml，并与预处理细胞在37℃下孵育3小时的方法，相对于使用活细胞的方法，显示出100%的敏感性和特异性。

相似文献

1
Escherichia coli adherence to HEp-2 cells with prefixed cells.用固定细胞检测大肠杆菌对HEp-2细胞的黏附
J Clin Microbiol. 1995 May;33(5):1414-7. doi: 10.1128/jcm.33.5.1414-1417.1995.
2
Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.基于聚合酶链式反应检测束状菌毛基因鉴定肠致病性大肠杆菌
J Clin Microbiol. 1995 May;33(5):1375-7. doi: 10.1128/jcm.33.5.1375-1377.1995.
3
Epithelial cell invasiveness of non-enteropathogenic serotypes of Escherichia coli.大肠杆菌非肠道致病性血清型的上皮细胞侵袭性
J Diarrhoeal Dis Res. 1993 Jun;11(2):101-4.
4
Colostrum and human milk inhibit localized adherence of enteropathogenic Escherichia coli to HeLa cells.初乳和母乳可抑制肠道致病性大肠杆菌对HeLa细胞的局部黏附。
Acta Paediatr. 1992 Mar;81(3):266-7. doi: 10.1111/j.1651-2227.1992.tb12219.x.
5
Methods for studying adhesion of diarrheagenic Escherichia coli.研究致泻性大肠杆菌黏附的方法。
Methods Enzymol. 1995;253:324-36. doi: 10.1016/s0076-6879(95)53028-2.
6
Enteroaggregative Escherichia coli strains among classical enteropathogenic Escherichia coli O serogroups.经典肠致病性大肠杆菌O血清群中的聚集性大肠杆菌菌株。
J Clin Microbiol. 2002 Sep;40(9):3540-1. doi: 10.1128/JCM.40.9.3540-3541.2002.
7
Bacterial clump formation at the surface of liquid culture as a rapid test for identification of enteroaggregative Escherichia coli.液体培养表面细菌团块形成作为鉴定肠聚集性大肠杆菌的快速检测方法。
J Clin Microbiol. 1993 May;31(5):1397-9. doi: 10.1128/jcm.31.5.1397-1399.1993.
8
Association of Escherichia coli HEp-2 adherence patterns with type and duration of diarrhoea.大肠杆菌HEp-2黏附模式与腹泻类型及持续时间的关联
Lancet. 1991 Feb 2;337(8736):262-4. doi: 10.1016/0140-6736(91)90868-p.
9
A study on some phenotypic virulence markers of enteropathogenic Escherichia coli.一项关于肠致病性大肠杆菌某些表型毒力标志物的研究。
Indian J Med Res. 2001 Sep;114:95-8.
10
Adhesive properties of enteropathogenic Escherichia coli isolated from infants with acute diarrhea in Africa.从非洲急性腹泻婴儿中分离出的肠致病性大肠杆菌的黏附特性。
Eur J Clin Microbiol Infect Dis. 1989 Nov;8(11):979-83. doi: 10.1007/BF01967569.

引用本文的文献

1
Effects of Variety and Postharvest Handling Practices on Microbial Population at Different Stages of the Value Chain of Fresh Tomato in Western Terai of Nepal.不同品种及产后处理措施对尼泊尔西部特莱低地新鲜番茄价值链不同阶段微生物种群的影响。
Biomed Res Int. 2017;2017:7148076. doi: 10.1155/2017/7148076. Epub 2017 Oct 12.
2
Atypical enteropathogenic Escherichia coli strains form biofilm on abiotic surfaces regardless of their adherence pattern on cultured epithelial cells.非典型肠致病性大肠杆菌菌株无论其在培养的上皮细胞上的黏附模式如何，都会在非生物表面形成生物膜。
Biomed Res Int. 2014;2014:845147. doi: 10.1155/2014/845147. Epub 2014 May 6.
3
Laboratory investigation of enteroaggregative Escherichia coli O untypeable:H10 associated with a massive outbreak of gastrointestinal illness.与大规模胃肠道疾病暴发相关的不可分型的肠聚集性大肠杆菌O:H10的实验室调查。
J Clin Microbiol. 1997 Oct;35(10):2546-50. doi: 10.1128/jcm.35.10.2546-2550.1997.

本文引用的文献

1
Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines.兔源和人源肠致病性大肠杆菌在猪和兔肠道中的黏附和损伤作用
Infect Immun. 1983 Sep;41(3):1340-51. doi: 10.1128/iai.41.3.1340-1351.1983.
2
Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.肠致病性大肠杆菌对HeLa细胞的独特黏附模式。
Infect Immun. 1984 Aug;45(2):534-6. doi: 10.1128/iai.45.2.534-536.1984.
3
Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa.肠致病性大肠杆菌对人肠道肠上皮细胞和培养的人肠黏膜的黏附作用。
Infect Immun. 1987 Jan;55(1):69-77. doi: 10.1128/iai.55.1.69-77.1987.
4
Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease.肠黏附-聚集性大肠杆菌的特性研究，腹泻病的一种假定病原体
J Infect Dis. 1988 Jul;158(1):70-9. doi: 10.1093/infdis/158.1.70.
5
Plasmid-mediated factors conferring diffuse and localized adherence of enteropathogenic Escherichia coli.质粒介导的赋予肠致病性大肠杆菌弥漫性和局限性黏附的因子。
Infect Immun. 1985 May;48(2):378-83. doi: 10.1128/iai.48.2.378-383.1985.
6
Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli.细菌黏附于组织培养细胞部位的肌动蛋白积累：一种针对肠致病性和肠出血性大肠杆菌的新型诊断测试的基础
Infect Immun. 1989 Apr;57(4):1290-8. doi: 10.1128/iai.57.4.1290-1298.1989.
7
Comparison of two assay methods for patterns of adherence to HEp-2 cells of Escherichia coli from patients with diarrhea.两种检测腹泻患者大肠杆菌对HEp-2细胞黏附模式的方法比较。
J Clin Microbiol. 1990 May;28(5):882-5. doi: 10.1128/jcm.28.5.882-885.1990.
8
An ELISA for the detection of localized adherent classic enteropathogenic Escherichia coli serogroups.一种用于检测局限性黏附性经典肠致病性大肠杆菌血清群的酶联免疫吸附测定法。
J Infect Dis. 1991 Nov;164(5):986-9. doi: 10.1093/infdis/164.5.986.
9
Escherichia coli O128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescent-actin staining test but do not hybridize with an enteropathogenic E. coli adherence factor probe.来自腹泻婴儿的大肠杆菌O128菌株通常在荧光肌动蛋白染色试验中表现出局部黏附及阳性反应，但不与致病性大肠杆菌黏附因子探针杂交。
Infect Immun. 1991 Apr;59(4):1569-71. doi: 10.1128/iai.59.4.1569-1571.1991.
10
Comparison of a modified adherence assay with existing assay methods for identification of enteroaggregative Escherichia coli.一种改良的黏附试验与现有用于鉴定肠集聚性大肠杆菌的试验方法的比较。
J Clin Microbiol. 1992 Jun;30(6):1614-6. doi: 10.1128/jcm.30.6.1614-1616.1992.