Pavitt G D
Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Biochem Soc Trans. 2005 Dec;33(Pt 6):1487-92. doi: 10.1042/BST0331487.
eIF2B (eukaryotic initiation factor 2B) is a multisubunit protein that is required for protein synthesis initiation and its regulation in all eukaryotic cells. Mutations in eIF2B have also recently been found to cause a fatal human disease called CACH (childhood ataxia with central nervous system hypomyelination) or VWM (vanishing white matter disease). This review provides a general background to translation initiation and mechanisms known to control eIF2B function, before describing molecular genetic and biochemical analysis of eIF2B structure and function, integrating work from studies of the yeast and mammalian eIF2B proteins.
真核生物起始因子2B（eIF2B）是一种多亚基蛋白质，在所有真核细胞中，蛋白质合成起始及其调控过程都需要它。最近还发现，eIF2B的突变会导致一种致命的人类疾病，称为儿童共济失调伴中枢神经系统髓鞘形成低下（CACH）或消失性白质病（VWM）。在描述eIF2B结构和功能的分子遗传学及生化分析之前，本综述先介绍翻译起始的一般背景以及已知的控制eIF2B功能的机制，并整合来自酵母和哺乳动物eIF2B蛋白研究的成果。
