Maderia Melissa, Wu Justin, Bax Ad, Shenoy Shilpa, O'Keefe Barry, Marquez Victor E, Barchi Joseph J
Laboratory of Medicinal Chemistry, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA.
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):687-90. doi: 10.1081/ncn-200060256.
DNA dodecamers modified with nucleotide building blocks based on a bicyclo[3. 1.0]hexane system that effectively locks the ribose template into an RNA-like or North (N) conformation were analyzed by various biophysical techniques including high field nuclear magnetic resonance (NMR). Replacement of either one or both of the center thymidines in the Dickerson Drew dodecamer (CGCGAATTCGCG) caused a progressive shift in the bending propensity of the double helix as shown by a newly developed rapid technique that compares the residual dipolar coupling (RDC) values of the modified duplexes with those previously determined for the native DNA.
基于双环[3.1.0]己烷系统的核苷酸构建块修饰的DNA十二聚体，能有效地将核糖模板锁定为RNA样或North（N）构象，通过包括高场核磁共振（NMR）在内的各种生物物理技术进行了分析。迪克森·德鲁十二聚体（CGCGAATTCGCG）中一个或两个中心胸腺嘧啶的替换，导致双螺旋弯曲倾向逐渐改变，这是通过一种新开发的快速技术显示的，该技术将修饰双链体的剩余偶极耦合（RDC）值与先前测定的天然DNA的RDC值进行比较。
