在2'-脱氧鸟苷的C8位带有芳胺修饰的寡核苷酸的合成。
Synthesis of oligonucleotides bearing an arylamine modification in the C8-position of 2'-deoxyguanosine.
作者信息
Meier C, Gräsl S, Detmer I, Marx A
机构信息
Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, Hamburg D-20146, Germany.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):691-4. doi: 10.1081/ncn-200060267.
PMID:16248016
Abstract
C8-Arylamine-dG adducts were converted into their corresponding 5-O-DMTr-3'-O-phosphoramidite-C8-arylamine-dG derivatives. These compounds were used for the automated synthesis of site-specifically modified oligonucleotides. The oligonucleotides were studied for their CD properties, Tm values, and their effects on primer extension assays using human DNA-polymerase beta.
摘要
C8-芳胺-dG加合物被转化为其相应的5'-O-二甲氧基三苯甲基-3'-O-亚磷酰胺-C8-芳胺-dG衍生物。这些化合物用于位点特异性修饰寡核苷酸的自动化合成。对这些寡核苷酸的圆二色性性质、熔解温度值以及它们对使用人DNA聚合酶β的引物延伸测定的影响进行了研究。
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