由c8-芳胺嘌呤加合物位点特异性损伤的DNA链的合成及其对各种DNA聚合酶的影响。

Synthesis of DNA strands site-specifically damaged by c8-arylamine purine adducts and effects on various DNA polymerases.

作者信息

Böge Nicolas, Jacobsen Maike I, Szombati Zita, Baerns Sabrina, Di Pasquale Francesca, Marx Andreas, Meier Chris

机构信息

Organic Chemistry, Department of Chemistry, Faculty of Science, University of Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.

出版信息

Chemistry. 2008;14(35):11194-208. doi: 10.1002/chem.200800979.

DOI:10.1002/chem.200800979

PMID:18988239

Abstract

C8-Arylamine-dG and C8-arylamine-dA adducts have been prepared using palladium cross-coupling chemistry. These adducts were subsequently converted into the corresponding 5'-O-DMTr-C8-arylamine-3'-O-phosphoramidites and then used for the automated synthesis of different site-specifically modified oligonucleotides. These "damaged" oligonucleotides have been characterized by ESI-MS, UV thermal stability assays, and circular dichroism, and they have been used in EcoRI assays as well as in primer extension studies using various DNA polymerases.

摘要

已使用钯交叉偶联化学方法制备了C8-芳胺-dG和C8-芳胺-dA加合物。这些加合物随后被转化为相应的5'-O-二甲氧基三苯甲基-C8-芳胺-3'-O-亚磷酰胺，然后用于自动合成不同位点特异性修饰的寡核苷酸。这些“受损”寡核苷酸已通过电喷雾电离质谱（ESI-MS）、紫外热稳定性测定和圆二色性进行了表征，并且已用于EcoRI测定以及使用各种DNA聚合酶的引物延伸研究中。