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[从牛和宠物中分离产志贺毒素大肠杆菌]

[Isolation of verocytotoxin-producing Escherichia coli from cattle and pets].

作者信息

Tanaka H, Kondo R, Nishiuchi C, Matsuda H, Kimura M, Memida K, Kikuchi M, Tsukamoto T, Yamasaki S, Lin Z

机构信息

Iyo Public Health Center, Ehime.

出版信息

Kansenshogaku Zasshi. 1992 Apr;66(4):448-55. doi: 10.11150/kansenshogakuzasshi1970.66.448.

Abstract

During a three-year period (1987 & 1990), a comprehensive attempt was made to isolate verocytotoxin-producing Escherichia coli (VTEC) from 96 bovine, 89 porcine, 67 canine and 18 feline cases suffering from gastroenteritis. VTEC were isolated from 11 cows and 1 cat. Bead-ELISA and oligonucteotide probes were used to type the verotoxins (VT) and it was found that the VTEC strains from cows produced not only the currently recognized VT1, VT2 and VT2vh but also produced two new VT's tentatively designated as VTx and VTy. The strain from the cat produced VTy. Serotyping of the strains revealed that some animal strains belonged to similar serotypes as those isolated from human patients with hemorrhagic colitis and hemolytic uremic syndrome. Cattle, especially cows, and domestic pets apparently are reservoirs of VTEC and probable sources of infection in our country as has been previously documented in Canada and USA.

摘要

在三年期间(1987年至1990年),我们进行了全面的尝试,从96例患肠胃炎的牛、89例猪、67例犬和18例猫病例中分离产志贺毒素大肠杆菌(VTEC)。从11头牛和1只猫中分离出了VTEC。使用珠式酶联免疫吸附测定法(Bead - ELISA)和寡核苷酸探针来鉴定志贺毒素(VT)类型,结果发现来自牛的VTEC菌株不仅产生目前公认的VT1、VT2和VT2vh,还产生了两种新的暂时命名为VTx和VTy的VT。来自猫的菌株产生VTy。对这些菌株进行血清分型显示,一些动物菌株与从患有出血性结肠炎和溶血尿毒综合征的人类患者中分离出的菌株属于相似的血清型。牛,尤其是奶牛,以及家养宠物显然是VTEC的储存宿主,并且如加拿大和美国之前所记录的那样,很可能是我国的感染源。

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