编码不同O:K:H血清型的产志贺样毒素牛源肠出血性大肠杆菌（VTEC）菌株中志贺样毒素的基因。

Genes coding for Shiga-like toxins in bovine verotoxin-producing Escherichia coli (VTEC) strains belonging to different O:K:H serotypes.

作者信息

Blanco M, Blanco J, Blanco J E, González E A, Gomes T A, Zerbini L F, Yano T, de Castro A F

机构信息

Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.

出版信息

Vet Microbiol. 1994 Nov;42(2-3):105-10. doi: 10.1016/0378-1135(94)90010-8.

DOI:10.1016/0378-1135(94)90010-8

PMID:7886925

Abstract

Forty-six verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrhoeic and healthy calves in Spain were examined for DNA sequences homologous to genes for verotoxins (VT1 and VT2) and enterotoxins (LT-I, LT-II, STaH, STaP and STb). Hybridisation showed that 26 (57%) of VTEC strains carried VT1 genes, 13 (28%) possessed VT2 genes, and 7 (15%) carried both VT1 and VT2 genes. No VTEC strains hybridised with DNA probes for enterotoxins. A correlation was found between the serotype and type of VT produced. Thus, all strains of serotypes O26:K-:H11 (13 strains), O103:K-:H2 (3 strains) and O128:K?:H- (4 strains) hybridised with the VT1 probe only, whereas all strains of serotypes O4:K-:H4 (3 strains) and O113:K-:H21 (4 strains) were positive with the VT2 probe only. By contrast, O81:K?:H28 (2 strains) and O157:K-:H- (2 strains) strains hybridised with both VT1 and VT2 probes. One strain of serotype O157:K-:H7 was VT2 positive.

摘要

对从西班牙腹泻和健康小牛中分离出的46株产志贺毒素大肠杆菌（VTEC）菌株进行检测，以寻找与志贺毒素（VT1和VT2）及肠毒素（LT-I、LT-II、STaH、STaP和STb）基因同源的DNA序列。杂交结果显示，26株（57%）VTEC菌株携带VT1基因，13株（28%）拥有VT2基因，7株（15%）同时携带VT1和VT2基因。没有VTEC菌株与肠毒素的DNA探针杂交。发现血清型与所产VT类型之间存在相关性。因此，所有血清型为O26:K-:H11的菌株（13株）、O103:K-:H2的菌株（3株）和O128:K?:H-的菌株（4株）仅与VT1探针杂交，而所有血清型为O4:K-:H4的菌株（3株）和O113:K-:H21的菌株（4株）仅与VT2探针呈阳性反应。相比之下，O81:K?:H28的菌株（2株）和O157:K-:H-的菌株（2株）与VT1和VT2探针均杂交。一株血清型为O157:K-:H7的菌株VT2呈阳性。