Happe M, Teathera R M, Overath P, Knobling A, Oesterhelt D
Max-Planck-Institut für Biologie, D74 Tübingen, GFR.
Biochim Biophys Acta. 1977 Mar 1;465(2):415-20. doi: 10.1016/0005-2736(77)90092-x.
The reconstitution of proton pumping activity in proteoliposomes formed by brief sonication of purple membrane and lipid dispersions was studied as a function of pH. Proteoliposomes reconstituted using cardiolipin showed light-dependent proton extrusion when formed at a pH below 2.75 and proton uptake when formed above pH 2.75. Several other acidic lipids including halobacterial lipids behaved similarly. The experiments suggest that the degree of dissociation of the lipid phosphate groups determines the preferential orientation of bacteriorhodopsin in reconstituted proteoliposomes.
研究了通过短暂超声处理紫膜和脂质分散体形成的蛋白脂质体中质子泵活性的重建与pH值的关系。使用心磷脂重建的蛋白脂质体在pH低于2.75时形成表现出光依赖的质子外排，在pH高于2.75时形成表现出质子摄取。其他几种酸性脂质，包括嗜盐菌脂质，表现类似。实验表明，脂质磷酸基团的解离程度决定了细菌视紫红质在重建的蛋白脂质体中的优先取向。
