Jack Clifford R, Wengenack Thomas M, Reyes Denise A, Garwood Michael, Curran Geoffrey L, Borowski Bret J, Lin Joseph, Preboske Gregory M, Holasek Silvina S, Adriany Gregor, Poduslo Joseph F
Department of Radiology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
J Neurosci. 2005 Oct 26;25(43):10041-8. doi: 10.1523/JNEUROSCI.2588-05.2005.
The ability to detect individual Alzheimer's amyloid plaques in vivo by magnetic resonance microimaging (MRI) should improve diagnosis and also accelerate discovery of effective therapeutic agents for Alzheimer's disease (AD). Here, we perform in vivo and ex vivo MRI on double transgenic AD mice as well as wild-type mice at varying ages and correlate these with thioflavin-S and iron staining histology. Quantitative counts of individual plaques on MRI increase with age and correlate with histologically determined plaque burden. Plaques 20 microm in diameter can be detected in AD mice as young as 3 months of age with ex vivo MRI. Plaques 35 microm in diameter can be detected by 9 months of age with in vivo MRI. In vivo MRI of individual Alzheimer's amyloid plaques provides a noninvasive estimate of plaque burden in transgenic AD mice that might be useful in assessing the efficacy of amyloid reduction therapies.
通过磁共振显微成像(MRI)在体内检测单个阿尔茨海默病淀粉样斑块的能力应能改善诊断,并加速阿尔茨海默病(AD)有效治疗药物的发现。在此,我们对不同年龄的双转基因AD小鼠以及野生型小鼠进行了体内和体外MRI检查,并将这些结果与硫黄素-S和铁染色组织学结果相关联。MRI上单个斑块的定量计数随年龄增加而增加,并与组织学确定的斑块负担相关。直径20微米的斑块在3个月大的AD小鼠中通过体外MRI即可检测到。直径35微米的斑块在9个月大时通过体内MRI即可检测到。单个阿尔茨海默病淀粉样斑块的体内MRI提供了转基因AD小鼠斑块负担的非侵入性估计,这可能有助于评估淀粉样蛋白减少疗法的疗效。