Bordenstein Seth, Rosengaus Rebeca B
The Marine Biological Laboratory, The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, MA 02543, USA.
Curr Microbiol. 2005 Dec;51(6):393-8. doi: 10.1007/s00284-005-0084-0. Epub 2005 Oct 25.
Wolbachia are one of the most abundant groups of bacterial endosymbionts in the biosphere. Interest in these heritable microbes has expanded with the discovery of wider genetic diversity in under-sampled host species. Here, we report on the putative discovery of a new genetic lineage, denoted super group H, which infects the Isopteran species Zootermopsis angusticollis and Z. nevadensis. Evidence for this novel super group is based on portions of new Wolbachia gene sequences from each species spanning 3.5 kilobases of DNA and the following genes: 16S rDNA, dnaA, gltA, groEL, and ftsZ. Single-gene and concatenated maximum likelihood phylogenies establish this new super group and validate the positioning of the other Wolbachia super groups. This discovery is the first example of a termite Wolbachia that is highly divergent from the Isopteran Wolbachia previously described in super group F. This study highlights the importance of multilocus approaches to resolving Wolbachia super group relationships. It also suggests that surveys of Wolbachia in more earlier-originating (and under-sampled) groups of arthropods are more apt to reveal novel genetic diversity.
沃尔巴克氏体是生物圈中最丰富的细菌内共生体群体之一。随着在抽样不足的宿主物种中发现更广泛的遗传多样性，对这些可遗传微生物的兴趣也在增加。在这里，我们报告了一个新的遗传谱系（命名为超级组H）的推定发现，它感染等翅目物种窄颈大白蚁（Zootermopsis angusticollis）和内华达大白蚁（Z. nevadensis）。这一新超级组的证据基于来自每个物种的沃尔巴克氏体新基因序列的部分片段，这些片段跨越3.5千碱基的DNA以及以下基因：16S rDNA、dnaA、gltA、groEL和ftsZ。单基因和串联最大似然系统发育树确定了这个新的超级组，并验证了其他沃尔巴克氏体超级组的定位。这一发现是白蚁沃尔巴克氏体与先前在超级组F中描述的等翅目沃尔巴克氏体高度不同的第一个例子。这项研究强调了多位点方法在解决沃尔巴克氏体超级组关系方面的重要性。它还表明，对更多起源较早（且抽样不足）的节肢动物群体中的沃尔巴克氏体进行调查更有可能揭示新的遗传多样性。
