Magee J T, Hindmarch J M, Duerden B I, Goodwin L
Department of Experimental and Clinical Microbiology, University of Sheffield Medical School.
J Med Microbiol. 1992 Jul;37(1):56-61. doi: 10.1099/00222615-37-1-56.
Clinical (66) and reference (5) strains of pigmented gram-negative anaerobic bacilli, identified as Prevotella intermedia (47), Pr. melaninogenica (1), Pr. corpora (8), Porphyromonas asaccharolyticus (12), P. endodontalis (1) and P. gingivalis (2), were examined by pyrolysis mass spectrometry (PMS) and in conventional tests. Numerical classification based on conventional test reaction patterns (CTRPs) resolved five clusters, four comprising strains identified as Pr. intermedia, Pr. corpora, Pr. melaninogenica, and P. gingivalis respectively, and one comprising strains identified as P. asaccharolyticus and P. endodontalis. Numerical classification based on PMS showed a similar division, with decreasing homogeneity of chemical composition in the order Pr. intermedia, Pr. corpora, P. asaccharolyticus, which agreed with the order of homogeneity in CTRPs. PMS clusters corresponding to the genus Porphyromonas were clearly distinct from those of the genus Prevotella. PMS and CTRP classification disagreed on cluster membership for six strains. PMS identification from blind challenge sets was in agreement with conventional identification for 64 of 67 strains.
对临床（66株）和参考（5株）的色素革兰氏阴性厌氧杆菌菌株进行了热解质谱分析（PMS）和常规检测，这些菌株被鉴定为中间普氏菌（47株）、产黑色素普氏菌（1株）、人体普氏菌（8株）、不解糖卟啉单胞菌（12株）、牙髓卟啉单胞菌（1株）和牙龈卟啉单胞菌（2株）。基于常规测试反应模式（CTRP）的数值分类解析出五个聚类，其中四个聚类分别包含被鉴定为中间普氏菌、人体普氏菌、产黑色素普氏菌和牙龈卟啉单胞菌的菌株，另一个聚类包含被鉴定为不解糖卟啉单胞菌和牙髓卟啉单胞菌的菌株。基于PMS的数值分类显示出类似的划分，化学组成的同质性从高到低依次为中间普氏菌、人体普氏菌、不解糖卟啉单胞菌，这与CTRP中的同质性顺序一致。与卟啉单胞菌属相对应的PMS聚类与普氏菌属的聚类明显不同。在六个菌株的聚类归属上，PMS和CTRP分类存在分歧。从盲测集中通过PMS鉴定的结果与67株中的64株的常规鉴定结果一致。
