Murdoch D A, Magee J T
Department of Microbiology, Southmead Hospital, Bristol.
J Med Microbiol. 1995 Aug;43(2):148-55. doi: 10.1099/00222615-43-2-148.
Clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (PMS). Numerical classifications based upon conventional test reaction patterns (CTRPs) and PMS showed 27 and 22 clusters, respectively. Cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species Peptostreptococcus anaerobius, P. heliotrinreducens, P. hydrogenalis, P. indolicus, P. lactolyticus, P. magnus, P. micros and Peptococcus niger. Strains of P. prevotii and P. tetradius clustered together in a heterogeneous group of saccharolytic organisms. However, strains previously identified as P. asaccharolyticus were divided into three distinct groups in PMS, two of which differed only in indole-associated pyrolysis products. A further four groups and several single-member clusters were distinct from these species. PMS data supported the validity of identification by pre-formed enzyme profiles and confirmed that Hare group III is synonymous with P. hydrogenalis, Hare group IV with P. magnus, and the "ADH group" with P. vaginalis. There is clearly a need for a taxonomic revision of the genus Peptostreptococcus, which probably encompasses several generic groups.
对革兰氏阳性厌氧球菌的临床菌株（101株）和收集菌株（26株）进行了常规试验和热解质谱分析（PMS）。基于常规试验反应模式（CTRPs）和PMS的数值分类分别显示出27个和22个聚类。两种分类中聚类成员的交叉列表显示出极好的相关性，联合分类显示出与目前公认的厌氧消化链球菌、嗜黑麦草消化链球菌、产氢消化链球菌、产吲哚消化链球菌、溶乳糖消化链球菌、大消化链球菌、微小消化链球菌和黑色消化球菌物种相对应的清晰组别。普氏消化链球菌和四联消化链球菌菌株聚集在一群异质性的糖分解生物中。然而，先前鉴定为非糖分解消化链球菌的菌株在PMS中被分为三个不同的组，其中两组仅在与吲哚相关的热解产物上有所不同。另外四个组和几个单成员聚类与这些物种不同。PMS数据支持通过预先形成的酶谱进行鉴定的有效性，并证实黑尔第三组与产氢消化链球菌同义，黑尔第四组与大消化链球菌同义，以及“ADH组”与阴道消化链球菌同义。显然有必要对消化链球菌属进行分类学修订，该属可能包含几个类属群。
