Hiltunen Y, Ala-Korpela M, Jokisaari J, Eskelinen S, Kiviniitty K
Department of Physics, University of Oulu, Finland.
Magn Reson Med. 1992 Jul;26(1):89-99. doi: 10.1002/mrm.1910260110.
The usefulness of proton NMR spectroscopy of human blood plasma for cancer research has been extensively studied in recent years. Two main starting points have been offered by Fossel et al. (N. Engl. J. Med. 315, 1369 (1986)) and Mountford et al. (FEBS Lett. 203, 164 (1986)). In this work the experimental proton NMR spectra of blood plasma were analyzed with the aid of the multivariate lineshape fitting method. An appropriate model structure, in terms of the various lipoprotein (VLDL, LDL, and HDL) signals, for the methylene region was used. Neonates, healthy adults, and adults with nonmalignant and malignant tumors were studied. The linewidth of the methylene region was found to be linearly dependent on the relative concentrations of the lipoproteins. The correlation coefficient was -0.89 (P less than 0.001) for VLDL and 0.88 (P less than 0.001) for HDL. A correlation between VLDL concentration and age, 0.76 (P less than 0.001), was also established. VLDL was modeled using two components. The half-linewidth of the lower field component was slightly elevated for the adults with large metastases. This might be in association with the fucose-containing proteolipid complex detected earlier in cancer cells or in sera of cancer patients. Some signals of this complex may fall in the same region of the spectra. The spectra for the neonates were indicated to be totally different from the adults. This and other related questions were explained by means of the model parameters and the relative concentrations of the lipoproteins VLDL, LDL, and HDL. The presented technique can be used as a rapid research tool for figuring out the relative concentrations of the lipoproteins in blood plasma and explaining the reasons behind the changes in the spectra.
近年来，人体血浆的质子核磁共振光谱在癌症研究中的实用性得到了广泛研究。福塞尔等人（《新英格兰医学杂志》315, 1369 (1986)）和芒特福德等人（《欧洲生物化学学会联合会快报》203, 164 (1986)）提出了两个主要出发点。在这项工作中，借助多变量线形拟合方法对血浆的实验质子核磁共振光谱进行了分析。针对亚甲基区域，使用了一个基于各种脂蛋白（极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白）信号的合适模型结构。对新生儿、健康成年人以及患有非恶性和恶性肿瘤的成年人进行了研究。发现亚甲基区域的线宽与脂蛋白的相对浓度呈线性相关。极低密度脂蛋白的相关系数为 -0.89（P小于0.001），高密度脂蛋白的相关系数为0.88（P小于0.001）。还建立了极低密度脂蛋白浓度与年龄之间的相关性，为0.76（P小于0.001）。极低密度脂蛋白使用两个成分进行建模。对于有大转移灶的成年人，低场成分的半高宽略有升高。这可能与早期在癌细胞或癌症患者血清中检测到的含岩藻糖的蛋白脂质复合物有关。该复合物的一些信号可能落在光谱的同一区域。结果表明新生儿的光谱与成年人的完全不同。通过模型参数以及脂蛋白极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白的相对浓度对这一问题及其他相关问题进行了解释。所提出的技术可作为一种快速研究工具，用于确定血浆中脂蛋白的相对浓度，并解释光谱变化背后的原因。
