Hiltunen Y, Ala-Korpela M, Jokisaari J, Eskelinen S, Kiviniitty K, Savolainen M, Kesäniemi Y A
Department of Physics, University of Oulu, Finland.
Magn Reson Med. 1991 Oct;21(2):222-32. doi: 10.1002/mrm.1910210207.
A lineshape fitting model was constructed for classifying the overlapping information in the 1H NMR spectrum of human blood plasma. A reliable assignment of the overlapping fatty acid (-CH2-)n and -CH3 resonances of the various lipoproteins (VLDL, very low density lipoprotein; LDL, low density lipoprotein; HDL high density lipoprotein) is introduced, and for the first time detailed characteristics (chemical shifts, half linewidths, and relative intensities) of the individual lipoprotein components were obtained directly from the whole plasma spectrum. This was achieved by combining the constructed lineshape fitting model and the proper 400 MHz proton NMR measurements from blood plasma of a healthy donor, from fractions of the different lipoproteins, and from plasma samples in which the lipoprotein fractions were separately added. The results suggest fair promise of future applications of the rapid and easy NMR analysis of lipoprotein distribution in various research and clinical situations.
构建了一种线形拟合模型，用于对人血浆的1H NMR谱中的重叠信息进行分类。引入了各种脂蛋白（极低密度脂蛋白、低密度脂蛋白、高密度脂蛋白）中重叠的脂肪酸（-CH2-）n和-CH3共振峰的可靠归属，首次直接从全血浆谱中获得了各脂蛋白组分的详细特征（化学位移、半峰宽和相对强度）。这是通过将构建的线形拟合模型与来自健康供体血浆、不同脂蛋白组分以及分别添加了脂蛋白组分的血浆样品的适当400 MHz质子NMR测量结果相结合来实现的。结果表明，在各种研究和临床情况下，快速简便的脂蛋白分布NMR分析具有良好的应用前景。
