Wilding P, Senior M B, Inubushi T, Ludwick M L
Department of Pathology, University of Pennsylvania, Philadelphia 19104-4283.
Clin Chem. 1988 Mar;34(3):505-11.
Water-suppressed proton nuclear magnetic resonance spectra were generated (by using 360 and 500 MHz systems) from human plasma and serum samples taken from 35 apparently healthy individuals, 52 patients with overt malignancies, and 37 patients with hypertriglyceridemia (triglycerides greater than 200 mg/dL or 2.26 mmol/L). The line widths from the lipoprotein-lipid methylene and methyl resonances at approximately 1.3 and 0.9 ppm were averaged by the method of Fossel et al. (N Engl J Med 1986;315:1369-76), but, contrary to their findings, we were unable to distinguish normal individuals from those with malignant tumors (e.g., mean +/- SD line width at 360 MHz: normal group = 32.9 +/- 3.6 Hz, malignant group = 28.3 +/- 4.9 Hz). The average line-width measurements (y), however, varied with the triglyceride content (x, mg/dL) of the plasma or serum as follows (logarithmic transformation of the data determined at 360 MHz and regression analysis): y = 110 (x-0.27). Data from both nonmalignant and malignant specimens fit this equation, the coefficient of correlation being -0.91. These findings suggest that considerable caution should be used in interpreting water-suppressed proton NMR spectra for cancer detection.
利用360兆赫和500兆赫系统，对35名表面健康的个体、52名患有明显恶性肿瘤的患者以及37名高甘油三酯血症患者（甘油三酯大于200毫克/分升或2.26毫摩尔/升）的人血浆和血清样本进行了水抑制质子核磁共振光谱分析。脂蛋白 - 脂质亚甲基和甲基共振在约1.3 ppm和0.9 ppm处的线宽，采用Fossel等人的方法（《新英格兰医学杂志》1986年；315:1369 - 76）进行平均，但与他们的研究结果相反，我们无法区分正常个体与患有恶性肿瘤的个体（例如，360兆赫时的平均±标准差线宽：正常组 = 32.9±3.6赫兹，恶性组 = 28.3±4.9赫兹）。然而，平均线宽测量值（y）随血浆或血清中甘油三酯含量（x，毫克/分升）的变化如下（对在360兆赫下测定的数据进行对数转换并进行回归分析）：y = 110（x - 0.27）。来自非恶性和恶性样本的数据均符合该方程，相关系数为 -0.91。这些发现表明，在解释用于癌症检测的水抑制质子核磁共振光谱时应格外谨慎。
