Yang Ben-Chao, Xiao Bing-Guang, Chen Xue-Jun, Shi Chun-Hai
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Yi Chuan. 2005 Sep;27(5):753-8.
Genetic diversity was assessed among 24 flue-cured tobacco varieties by ISSR (inter simple sequence repeats). A total of 100 ISSR primers were used to amplify the DNA from these varieties, of which 10 primers produced reproducible amplified products. Using polyacrylamide gel electrophoresis 208 bands were identified, of which 141 bands were polymorphic among the flue-cured tobacco varieties analyzed. Each primer produced 7-37 bands, the length of which ranged 200-2,400 bp. The ratio of polymorphic bands (PPB) was 67.79%. By cluster analysis based on ISSR markers using UPGMA, 24 varieties were divided into 5 major groups, in which the biggest group consisted of 12 varieties derived from Coker319. The genetic similarity index was 0.66-0.85 among 24 flue-cured tobacco varieties. Low genetic diversity among flue-cured tobacco varieties suggested that it is necessary to expand the genetic base of the flue-cured tobacco. 24 varieties could be distinguished by using 2 ISSR markers. The result also indicated that ISSR analysis was suitable for varietal identification and the study on genetic diversity of tobacco germplasm.
利用ISSR（简单重复序列区间）技术对24个烤烟品种的遗传多样性进行了评估。共使用100条ISSR引物对这些品种的DNA进行扩增，其中10条引物产生了可重复的扩增产物。通过聚丙烯酰胺凝胶电泳鉴定出208条带，在所分析的烤烟品种中，有141条带具有多态性。每条引物产生7 - 37条带，其长度范围为200 - 2400 bp。多态性条带比例（PPB）为67.79%。采用UPGMA法基于ISSR标记进行聚类分析，将24个品种分为5个主要类群，其中最大的类群由12个源自Coker319的品种组成。24个烤烟品种间的遗传相似性指数为0.66 - 0.85。烤烟品种间遗传多样性较低，表明有必要拓宽烤烟的遗传基础。利用2个ISSR标记可区分这24个品种。结果还表明，ISSR分析适用于烟草品种鉴定及种质遗传多样性研究。
