Wei Ji-Cheng, Qiu En-Jian, Guo Hui-Yan, Hao Ai-Ping, Chen Rong-Ping
Department of Biology, Mudanjiang Teachers College , Mudanjiang , P.R. China.
Laboratory of Breeding, Mudanjiang Tobacco Research Institute , Mudanjiang , P.R. China.
Biotechnol Biotechnol Equip. 2014 Mar 4;28(2):217-220. doi: 10.1080/13102818.2014.907651. Epub 2014 Jul 3.
A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymerase chain reaction (PCR) methods and T-A cloning. The sequences from common tobacco ( L.) and rustica tobacco ( L.) were analysed between the third intron and the fourth intron of the propylene alcohol dehydrogenase gene. The results showed that the alcohol dehydrogenase gene is a low-copy nuclear gene. The intron sequences have a combination of single nucleotide polymorphisms and length polymorphisms between common tobacco and rustica tobacco, which are suitable to identify the different germplasms. Furthermore, there are some single nucleotide polymorphism sites in the target sequence within common tobacco that can be used to distinguish intraspecific varieties.
基于烟草烯丙醇脱氢酶基因的cDNA序列设计了一对引物，用于扩增丙醇脱氢酶基因序列。使用传统的聚合酶链反应（PCR）方法和T-A克隆对所有内含子进行测序。分析了普通烟草（L.）和黄花烟草（L.）在丙醇脱氢酶基因的第三个内含子和第四个内含子之间的序列。结果表明，醇脱氢酶基因是一个低拷贝核基因。普通烟草和黄花烟草的内含子序列存在单核苷酸多态性和长度多态性的组合，适合用于鉴定不同的种质。此外，普通烟草的目标序列中存在一些单核苷酸多态性位点，可用于区分种内品种。
