基于SRAP和ISSR标记的党参遗传多样性研究

[Study on genetic diversity of Codonopsis tangshen by SRAP and ISSR markers].

作者信息

Chen Daxia, Peng Rui, Li Longyun, Sun Nianxi, Zhong Guoyue, Cai Yingfan

机构信息

Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Feb;34(3):255-9.

PMID:19445142

Abstract

OBJECTIVE

To study the genetic diversity of Codonopsis tangshen.

METHOD

Eighteen germplasmic resources of C. tangshen were analyzed by SRAP and ISSR molecular markers. The systematic diagram of genetic relationship was made by TREECONW software and clustered by UPGMA method.

RESULT

Twenty-nine SRAP primer combination amplified 329 bands with 266 (80.85%) polymorphic and 21 ISSR primers amplified 223 bands with 166 (74.44%) polymorphic. The average genetic similarity coefficient was 0.7121 (by SRAPs) and 0.7781 (by ISSRs). Both SRAP and ISSR analyses revealed a high level of genetic diversity in C. tangshen. By cluster analysis, the geographical distribution was not distinctive. The significant positive correlation between SRAPs and ISSRs was observed (r=0.802, P<0.01), although the dendrograms based on SRAP and ISSR markers were not all the same.

CONCLUSION

Different germplasms diversity of C. tangshen is high and SRAP and ISSR can be used in genetic diversity study of C. tangshen.

摘要

目的

研究党参的遗传多样性。

方法

采用SRAP和ISSR分子标记对18份党参种质资源进行分析。利用TREECONW软件绘制遗传关系系统图，并采用UPGMA法进行聚类。

结果

29对SRAP引物组合扩增出329条带，其中多态性条带266条（80.85%）；21条ISSR引物扩增出223条带，其中多态性条带166条（74.44%）。SRAP分析的平均遗传相似系数为0.7121，ISSR分析的平均遗传相似系数为0.7781。SRAP和ISSR分析均表明党参具有较高的遗传多样性。聚类分析结果显示，其地理分布不具有明显的特异性。尽管基于SRAP和ISSR标记构建的聚类图不完全相同，但SRAP与ISSR分析结果之间存在显著正相关（r = 0.802，P < 0.01）。