Mohamed Abdel-Fattah, Ali Hasan Abeer Gafar, Hamamy Mahmoud Ismail, Abdel-Sattar Essam
Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Med Sci Monit. 2005 Nov;11(11):BR426-31.
Since phenolic compounds have been reported as effective antioxidants, this study was designed to assess the hepatoprotective and antioxidant activities of the chloroformic extract of the resinous exudate and its phenolic constituents obtained from the stems of Eucalyptus maculata.
MATERIAL/METHODS: The chloroformic extract and pure phenolic isolates were evaluated for their antioxidant and hepatoprotective properties in mice and rats based on biochemical changes in serum and tissues as well as pathological changes in the liver and spleen.
Acetaminophen (ACP) at a dose of 1 g/kg body weight produced 100% mortality in mice, while pretreatment of animals with the chloroformic extract (125 and 250 mg/kg) protected against the moralities by 66%. Pretreatment of rats with either the chloroformic extract (250 mg/kg) or any of the pure isolates (20 mg/kg) significantly reduced the increase in serum level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) produced by ACP (640 mg/kg). Pretreatment of animals with the chloroformic extract or its isolates also protected against ascorbic acid depletion in serum and kidney tissues induced by oral administration of paraquat (PQ) without modifying the serum level of glutathione (GSH) and glycogen content in liver tissue.
The phenolic content of the chloroformic extract and the pure isolates produced an antioxidant activity which may be due to the formation of stable phenoxyl radical in addition to its effect through vitamin C.
由于酚类化合物已被报道为有效的抗氧化剂，本研究旨在评估从斑皮桉茎中获得的树脂渗出物的氯仿提取物及其酚类成分的肝脏保护和抗氧化活性。
材料/方法：基于血清和组织中的生化变化以及肝脏和脾脏的病理变化，对氯仿提取物和纯酚类分离物在小鼠和大鼠中的抗氧化和肝脏保护特性进行了评估。
对乙酰氨基酚（ACP）以1 g/kg体重的剂量给药使小鼠死亡率达到100%，而用氯仿提取物（125和250 mg/kg）对动物进行预处理可使死亡率降低66%。用氯仿提取物（250 mg/kg）或任何一种纯分离物（20 mg/kg）对大鼠进行预处理，可显著降低由ACP（640 mg/kg）引起的血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平的升高。用氯仿提取物或其分离物对动物进行预处理，还可防止口服百草枯（PQ）诱导的血清和肾脏组织中抗坏血酸的消耗，而不会改变肝脏组织中谷胱甘肽（GSH）的血清水平和糖原含量。
氯仿提取物和纯分离物中的酚类成分产生了抗氧化活性，这可能是由于除了通过维生素C发挥作用外，还形成了稳定的苯氧自由基。
