Bioelectrochemistry Laboratory, Chemical and Pharmaceutical Sciences Faculty, University of Chile, P.O. Box 233, Santiago 1, Chile.
Talanta. 2010 Jun 30;82(1):398-403. doi: 10.1016/j.talanta.2010.04.062. Epub 2010 May 7.
In this work, the electrochemical behavior and the analytical application of atomoxetine, a selective noradrenaline reuptake inhibitor, are studied. Atomoxetine, studied by differential pulse voltammetry and cyclic voltammetry on a glassy carbon electrode, exhibited an anodic response in aqueous media with pH between 1.5 and 7. In non-aqueous medium (acetonitrile), the drug exhibited two irreversible oxidation peaks that are diffusion controlled. From chronocoulometric studies in acetonitrile, it was determined that each oxidation signal involves two and four electrons, respectively. For analytical purposes, a differential pulse voltammetry technique in 0.1 mol L(-1) perchloric acid was selected, which exhibited adequate figures of merit. The percent recovery was 96.6+/-1.2 and the detection and quantitation limits were 6.9 x 10(-5) and 1.0 x 10(-4) mol L(-1), respectively. Also, results indicate that excipients do not interfere with the oxidation signal of atomoxetine, which leads to the conclusion that the developed method is satisfactorily selective for atomoxetine quantification in pharmaceuticals with no prior separation or extraction necessary. Finally, the proposed voltammetric method was successfully applied to both the assay and the uniformity content of atomoxetine in capsules. For comparison, high-performance liquid chromatography analysis was also performed.
在这项工作中，研究了选择性去甲肾上腺素再摄取抑制剂阿托西汀的电化学行为及其分析应用。阿托西汀在玻碳电极上通过差分脉冲伏安法和循环伏安法进行研究，在 pH 值在 1.5 到 7 之间的水溶液中表现出阳极响应。在非水介质（乙腈）中，该药物表现出两个不可逆的氧化峰，这是扩散控制的。通过在乙腈中的计时库仑法研究，确定每个氧化信号分别涉及两个和四个电子。出于分析目的，选择了在 0.1 mol L(-1)高氯酸中的差分脉冲伏安技术，该技术表现出了足够的优良特性。回收率为 96.6+/-1.2，检测限和定量限分别为 6.9 x 10(-5)和 1.0 x 10(-4) mol L(-1)。此外，结果表明辅料不会干扰阿托西汀的氧化信号，这得出结论，所开发的方法对药物中阿托西汀的定量具有令人满意的选择性，无需事先分离或提取。最后，该提议的伏安法成功地应用于胶囊中阿托西汀的含量测定和均匀度。为了进行比较，还进行了高效液相色谱分析。
