Uchida Sae, Kagitani Fusako, Hotta Harumi, Hanada Tomoko, Aikawa Yoshihiro
Department of the Autonomic Nervous System, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173-0015 Japan.
Jpn J Physiol. 2005 Oct;55(5):265-77. doi: 10.2170/jjphysiol.R2133. Epub 2005 Nov 1.
The reflex effects of noxious mechanical stimulation of a hindpaw or abdominal skin on ovarian blood flow, and the reflex pathways involved in those responses were examined in anesthetized rats. Blood flow in the left ovary was measured using a laser Doppler flowmeter, and the activity of the left ovarian sympathetic nerve and mean arterial pressure (MAP) of the common carotid artery were recorded. Stimulation of the left or right hindpaw for 30 s produced marked increases in ovarian sympathetic nerve activity and MAP. Ovarian blood flow slightly decreased during the stimulation and then slightly increased after the stimulation. After the left ovarian sympathetic nerves were severed, the same stimulus produced a remarkable monophasic increase in ovarian blood flow that was explained by passive vasodilation due to a marked increase in MAP. After spinal transection at the third thoracic (T3) level, the responses of MAP, ovarian sympathetic nerve activity, and ovarian blood flow to hindpaw stimulation were nearly abolished. Stimulation of the abdomen at the right or left side for 30 s produced slight increases in ovarian sympathetic nerve activity and MAP. Ovarian blood flow slightly decreased during the stimulation and then slightly increased after the stimulation. After the ovarian sympathetic nerves were severed, the response of the ovarian blood flow changed to a monophasic increase due to an increase in MAP. After spinal transection, stimulation of the left abdomen produced a moderate increase in MAP, a remarkable increase in ovarian sympathetic nerve activity and a slight decrease in ovarian blood flow during the stimulation. In contrast, stimulation of the right abdomen produced a smaller response in ovarian sympathetic nerve activity during the stimulation while it increased the MAP to a similar degree. Ovarian blood flow slightly increased after the end of stimulation, which was explained as passive vasodilation due to the increase in MAP. In conclusion, stimulation of somatic afferents affects ovarian blood flow by inducing changes in ovarian sympathetic nerve activities and blood pressure. When stimulation was applied to a hindpaw whose segment of afferent input is far from the segment of the ovarian sympathetic nerves, it took a supraspinal reflex pathway. However, when stimulation was applied to the abdomen whose spinal segment of the afferent is close to the segment of the ovarian sympathetic nerve output, there are spinal segmental reflex pathways. The present results demonstrate that spinal reflexes depend on the laterality of the stimulus, while supraspinal reflexes do not depend on the laterality of the stimulus.
在麻醉大鼠中，研究了后爪或腹部皮肤的有害机械刺激对卵巢血流的反射作用以及这些反应所涉及的反射通路。使用激光多普勒血流仪测量左侧卵巢的血流，并记录左侧卵巢交感神经的活动和颈总动脉的平均动脉压（MAP）。刺激左侧或右侧后爪30秒会使卵巢交感神经活动和MAP显著增加。刺激期间卵巢血流略有下降，刺激后略有增加。切断左侧卵巢交感神经后，相同刺激会使卵巢血流出现明显的单相增加，这是由于MAP显著升高导致的被动血管舒张所致。在第三胸椎（T3）水平进行脊髓横断后，MAP、卵巢交感神经活动和卵巢血流对后爪刺激的反应几乎消失。刺激右侧或左侧腹部30秒会使卵巢交感神经活动和MAP略有增加。刺激期间卵巢血流略有下降，刺激后略有增加。切断卵巢交感神经后，卵巢血流的反应因MAP升高而变为单相增加。脊髓横断后，刺激左侧腹部会使刺激期间MAP适度增加、卵巢交感神经活动显著增加以及卵巢血流略有下降。相比之下，刺激右侧腹部在刺激期间卵巢交感神经活动的反应较小，而MAP升高程度相似。刺激结束后卵巢血流略有增加，这被解释为由于MAP升高导致的被动血管舒张。总之，躯体传入神经的刺激通过诱导卵巢交感神经活动和血压的变化来影响卵巢血流。当刺激施加于传入输入节段远离卵巢交感神经节段的后爪时，采用的是脊髓上反射通路。然而，当刺激施加于传入脊髓节段靠近卵巢交感神经输出节段的腹部时，则存在脊髓节段反射通路。目前的结果表明，脊髓反射取决于刺激的侧别，而脊髓上反射则不取决于刺激的侧别。
