Badenhorst Paul, Xiao Hua, Cherbas Lucy, Kwon So Yeon, Voas Matt, Rebay Ilaria, Cherbas Peter, Wu Carl
Laboratory of Molecular Cell Biology, National Cancer Institute, NIH, Bethesda, Maryland 20814, USA.
Genes Dev. 2005 Nov 1;19(21):2540-5. doi: 10.1101/gad.1342605.
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.
果蝇NURF是一种含有ISWI的ATP依赖性染色质重塑复合物，通过催化核小体滑动来调节转录。为了确定NURF在体内的基因靶点，我们对缺乏NURF特异性亚基NURF301的突变体进行了全基因组表达分析。令人惊讶的是，在数百个受NURF调节的基因中，有一大组蜕皮激素反应靶点。Nurf301基因敲除突变体不会经历幼虫到蛹的变态，并且还会增强蜕皮激素受体中的显性负性突变。此外，纯化的NURF以蜕皮激素依赖性方式结合EcR，表明它是核受体活性的直接效应物。NURF在哺乳动物中的保守性对类固醇信号传导具有广泛的影响。
