Chew G K, Cruickshank M E, Rooney P H, Miller I D, Parkin D E, Murray G I
Department of Gynaecology Oncology, Aberdeen Royal Infirmary, Foresterhill, Aberdeen, UK.
Br J Cancer. 2005 Nov 28;93(11):1301-4. doi: 10.1038/sj.bjc.6602855.
The impact of the success of organised cervical screening programme results in a steady decline of the incidence of squamous cell carcinoma of the cervix but a concomitant increase in the incidence of the less common histological subtypes, particularly adenocarcinoma of the cervix (ACC). Although Human papillomavirus (HPV) infection is believed to be a necessary cause of cervical cancer, its role in the pathogenesis of ACC is not well established. Established associations between oncogenic strains of HPV and ACC are based on molecular studies carried out on entire tumour block sections. In this study, the cervical adenocarcinoma cells of a 10-year cohort of women diagnosed with ACC were dissected using the PixCell II Laser Microdissecting System to detect the HPV 16 genome sequence using the real-time quantitative polymerase chain reaction to confirm the presence of HPV DNA within ACC cells. By coupling these two sophisticated techniques, the HPV DNA copy number cell could be calculated to investigate its role. The prevalence of HPV 16 infection in this cohort was 24%, which is significantly higher than the control group (chi(2), P=0.014). Women with ACC also had significantly higher HPV DNA copy number per cell compared to the control group (P=0.00007). Higher HPV DNA copy number is associated with risk of developing ACC.
有组织的宫颈癌筛查项目取得成功，其影响是子宫颈鳞状细胞癌的发病率稳步下降，但较罕见组织学亚型的发病率随之上升，尤其是子宫颈腺癌（ACC）。尽管人乳头瘤病毒（HPV）感染被认为是宫颈癌的必要病因，但其在ACC发病机制中的作用尚未明确。HPV致癌毒株与ACC之间已确立的关联是基于对整个肿瘤组织块切片进行的分子研究。在本研究中，使用PixCell II激光显微切割系统对10年间诊断为ACC的女性队列的宫颈腺癌细胞进行解剖，采用实时定量聚合酶链反应检测HPV 16基因组序列，以确认ACC细胞内HPV DNA的存在。通过结合这两种精密技术，可以计算HPV DNA拷贝数细胞，以研究其作用。该队列中HPV 16感染的患病率为24%，显著高于对照组（卡方检验，P = 0.014）。与对照组相比，ACC女性每细胞的HPV DNA拷贝数也显著更高（P = 0.00007）。较高的HPV DNA拷贝数与发生ACC的风险相关。
