急性心肌梗死患者中CD34(+)、CD117(+)、CXCR4(+)、c-met(+)干细胞的动员与左心室射血分数及血浆NT-proBNP水平相关。
Mobilization of CD34(+), CD117(+), CXCR4(+), c-met(+) stem cells is correlated with left ventricular ejection fraction and plasma NT-proBNP levels in patients with acute myocardial infarction.
作者信息
Wojakowski Wojciech, Tendera Michal, Zebzda Anna, Michalowska Anna, Majka Marcin, Kucia Magdalena, Maslankiewicz Katarzyna, Wyderka Rafal, Król Marek, Ochala Andrzej, Kozakiewicz Krystyna, Ratajczak Mariusz Z
机构信息
3rd Division of Cardiology, Silesian School of Medicine, 45-47 Ziołowa Street, 40-635 Katowice, Poland.
出版信息
Eur Heart J. 2006 Feb;27(3):283-9. doi: 10.1093/eurheartj/ehi628. Epub 2005 Nov 2.
AIMS
The aim of the study was to assess the correlation between the number of CD34(+), CD117(+), c-met(+), CXCR4(+) stem cells mobilized into peripheral blood, left ventricular ejection fraction (LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction (AMI).
METHODS AND RESULTS
43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early (<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49 (P=0.0012) for CD34(+) cells, r=0.48 (P=0.0018) for CXCR4(+) cells, r=0.45 (P=0.0043) for CD117(+) cells, and r=0.41 (P=0.01) for c-met(+) cells and negatively correlated with NT-proBNP levels on admission r=-0.35 (P=0.024) for CD34(+) cells, r=-0.42 (P=0.007) for CXCR4(+) cells, r=-0.33 (P=0.04). In patients with LVEF </=40%, the peak number of CD34(+), CXCR4(+), CD117(+), and c-met(+) stem cells was significantly lower when compared patients with LVEF >40%. The number of CXCR4(+) cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels (r=-0.37; P=0.029 and r=-0.45, P=0.02) and maximum activity of CK-MB (r=-0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found.
CONCLUSION
The mobilization of CD34(+), CD117(+), CXCR4(+), c-met(+) stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.
目的
本研究旨在评估急性心肌梗死（AMI）患者外周血中动员的CD34(+)、CD117(+)、c-met(+)、CXCR4(+)干细胞数量与左心室射血分数（LVEF）、NT-proBNP水平及心肌坏死标志物之间的相关性。
方法与结果
纳入43例ST段抬高型心肌梗死（STEMI）患者。使用流式细胞仪检测干细胞数量，使用酶联免疫吸附测定试剂盒检测NT-proBNP、SDF-1、G-CSF、VEGF、IL-6和HGF的浓度。AMI早期（<12小时）动员的干细胞数量与LVEF显著正相关：CD34(+)细胞r = 0.49（P = 0.0012），CXCR4(+)细胞r = 0.48（P = 0.0018），CD117(+)细胞r = 0.45（P = 0.0043），c-met(+)细胞r = 0.41（P = 0.01），且与入院时NT-proBNP水平负相关，CD34(+)细胞r = -0.35（P = 0.024），CXCR4(+)细胞r = -0.42（P = 0.007），r = -0.33（P = 0.04）。在LVEF≤40%的患者中，与LVEF>40%的患者相比，CD34(+)、CXCR4(+)、CD117(+)和c-met(+)干细胞的峰值数量显著降低。入院时和24小时后CXCR4(+)细胞数量与相应的心肌肌钙蛋白I水平（r = -0.37；P = 0.029和r = -0.45，P = 0.02）及肌酸激酶同工酶（CK-MB）的最大活性（r = -0.37；P = 0.021）负相关。未发现造血细胞因子水平与LVEF之间存在显著相关性。
结论
STEMI早期外周血中CD34(+)、CD117(+)、CXCR4(+)、c-met(+)干细胞的动员与LVEF正相关，与NT-proBNP水平及心肌坏死标志物负相关。
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