Walker C, Bode E, Boer L, Hansel T T, Blaser K, Virchow J C
Swiss Institute of Allergy and Asthma Research, Davos, Switzerland.
Am Rev Respir Dis. 1992 Jul;146(1):109-15. doi: 10.1164/ajrccm/146.1.109.
Activation of lymphocyte subpopulations was determined in conjunction with levels of cytokines in peripheral blood and bronchoalveolar lavage (BAL) of asthmatics. Allergic asthmatics had increased numbers of CD4+ IL-2R+ T cells in peripheral blood and BAL, and T-cell activation closely correlated with numbers of low-affinity IgE receptor (CD23) bearing B cells. In contrast, in nonallergic asthmatics both CD4+ and CD8+ T cells from blood and BAL had increased expression of IL-2R, HLA-DR, and VLA-1. Furthermore, in the nonallergic asthmatics CD8+ T cells were decreased in blood but increased in BAL. Cytokine levels were determined in BAL fluid and supernatants from purified peripheral blood T cells and enriched BAL lymphocyte preparations. Allergic asthmatics were characterized by increased levels of IL-4 and IL-5, and this elevated IL-4 contributed to the elevated IgE levels found in these allergic subjects. In contrast, nonallergic asthmatics had elevated levels of IL-2 and IL-5, with IL-2 contributing to T-cell activation. In both types of asthma, the close correlation of IL-5 levels with eosinophilia suggests that IL-5 is responsible for the characteristic eosinophilia of asthma. Thus, we provide evidence of distinct T-cell activation resulting in different spectra of cytokines in allergic and nonallergic asthma.
结合哮喘患者外周血和支气管肺泡灌洗（BAL）中的细胞因子水平，测定淋巴细胞亚群的激活情况。过敏性哮喘患者外周血和BAL中CD4⁺IL-2R⁺T细胞数量增加，但T细胞激活与携带低亲和力IgE受体（CD23）的B细胞数量密切相关。相比之下，在非过敏性哮喘患者中，血液和BAL中的CD4⁺和CD8⁺T细胞均有IL-2R、HLA-DR和VLA-1表达增加。此外，非过敏性哮喘患者血液中的CD8⁺T细胞减少，但BAL中的CD8⁺T细胞增加。测定了BAL液以及纯化外周血T细胞和富集BAL淋巴细胞制剂上清液中的细胞因子水平。过敏性哮喘患者的特征是IL-4和IL-5水平升高，而这种升高的IL-4导致了这些过敏患者中发现的IgE水平升高。相比之下，非过敏性哮喘患者的IL-2和IL-5水平升高，IL-2导致T细胞激活。在两种类型的哮喘中，IL-5水平与嗜酸性粒细胞增多密切相关，这表明IL-5是哮喘特征性嗜酸性粒细胞增多的原因。因此，我们提供了证据表明，在过敏性和非过敏性哮喘中，不同的T细胞激活导致了不同的细胞因子谱。
