Mukolwe S W, Kocan A A, Wyckoff J H
Department of Veterinary Parasitology, Oklahoma State University, Stillwater 74078.
Ann N Y Acad Sci. 1992 Jun 16;653:172-7. doi: 10.1111/j.1749-6632.1992.tb19641.x.
Sera from 223 randomly selected dogs and 489 white-tailed deer (Odocoileus virginianus) were tested for antibodies to Borrelia burgdorferi using an indirect kinetic ELISA. Dog samples were obtained in 1989 whereas deer samples were obtained between 1975 and 1990. Ten known negatives and two known positives from each group were run on each plate as controls. Samples showing mean mOD values above the mean of negatives + 3 SD were considered positive. Twenty-six dog (11.7%) and 22 deer (4.5%) samples were positive. Deer reactors were first detected among 1978 samples. Reactive deer were from central and eastern Oklahoma whereas reactive dogs were mostly from central Oklahoma. Confirmed human cases between 1986 and 1989 were distributed throughout the state, thus showing no correlation with either deer or dog results.
使用间接动力学酶联免疫吸附测定法（ELISA），对223只随机挑选的犬以及489只白尾鹿（弗吉尼亚鹿）的血清进行了针对伯氏疏螺旋体抗体的检测。犬的样本于1989年采集，而鹿的样本则在1975年至1990年期间采集。每组各10个已知阴性样本和2个已知阳性样本作为对照，在每块板上进行检测。平均光密度（mOD）值高于阴性样本平均值加3个标准差的样本被视为阳性。26只犬（11.7%）和22只鹿（4.5%）的样本呈阳性。鹿的阳性样本最早在1978年的样本中被检测到。有反应的鹿来自俄克拉何马州中部和东部，而有反应的犬大多来自俄克拉何马州中部。1986年至1989年期间确诊的人类病例分布在全州，因此与鹿或犬的检测结果均无相关性。
