Greene R T, Walker R L, Nicholson W L, Levine J F
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Vet Microbiol. 1991 Jan;26(1-2):179-90. doi: 10.1016/0378-1135(91)90054-j.
An enzyme-linked immunosorbent assay (ELISA) was compared to an indirect immunofluorescence assay (IFA) for detection of IgG antibodies to Borrelia burgdorferi in dog sera. The concordance of the two tests was 93.5% for sera from dogs from Maryland (n = 93), 98.0% for sera from dogs from North Carolina (n = 446), and 97.2% for the combined sample groups (n = 539). Twenty-five of the 27 samples with discordant or low positive results were tested, and showed immunoblot reactions to 1 to 10 different bands. Reaction patterns and intensity of the bands were quite variable, and did not explain a reason for the discordance.
将酶联免疫吸附测定（ELISA）与间接免疫荧光测定（IFA）进行比较，以检测犬血清中针对伯氏疏螺旋体的IgG抗体。来自马里兰州的犬血清（n = 93）的两种检测方法的一致性为93.5%，来自北卡罗来纳州的犬血清（n = 446）的一致性为98.0%，合并样本组（n = 539）的一致性为97.2%。对27份结果不一致或弱阳性的样本中的25份进行了检测，结果显示对1至10条不同条带出现免疫印迹反应。条带的反应模式和强度变化很大，无法解释不一致的原因。
