Campanha N H, Neppelenbroek K H, Spolidorio D M P, Spolidorio L C, Pavarina A C
Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University, São Paulo, Brazil.
Oral Dis. 2005 Nov;11(6):392-8. doi: 10.1111/j.1601-0825.2005.01135.x.
Candida dubliniensis is a recently described Candida species associated with oral candidosis that exhibits a high degree of phenotypic similarity to Candida albicans. However, these species show differences in levels of resistance to antimycotic agents and ability to cause infections. Therefore, accurate clinical identification of C. dubliniensis and C. albicans species is important in order to treat oral candidal infections. Phenotypic identification methods are easy-to-use procedures for routine discrimination of oral isolates in the clinical microbiology laboratory. However, C. dubliniensis may be so far underreported in clinical samples because most currently used identification methods fail to recognize this yeast. Phenotypic methods depend on growth temperature, carbon source assimilation, chlamydospore and hyphal growth production, positive or negative growth on special media and intracellular enzyme production, among others. In this review, some phenotypic methods are presented with a special emphasis on the discrimination of C. dubliniensis and C. albicans.
都柏林念珠菌是一种最近被描述的与口腔念珠菌病相关的念珠菌物种，它与白色念珠菌表现出高度的表型相似性。然而，这些物种在对抗真菌药物的耐药水平和引起感染的能力方面存在差异。因此，准确临床鉴定都柏林念珠菌和白色念珠菌对于治疗口腔念珠菌感染很重要。表型鉴定方法是临床微生物实验室中用于常规鉴别口腔分离株的易于使用的程序。然而，都柏林念珠菌在临床样本中的报道可能至今较少，因为目前大多数使用的鉴定方法无法识别这种酵母菌。表型方法取决于生长温度、碳源同化、厚垣孢子和菌丝生长产生、在特殊培养基上的生长阳性或阴性以及细胞内酶产生等。在本综述中，介绍了一些表型方法，并特别强调了都柏林念珠菌和白色念珠菌的鉴别。
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